2i6q
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2i6q.gif|left|200px]] | + | [[Image:2i6q.gif|left|200px]] |
| - | + | ||
| - | '''Complement component C2a''' | + | {{Structure |
| + | |PDB= 2i6q |SIZE=350|CAPTION= <scene name='initialview01'>2i6q</scene>, resolution 2.100Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=MLI:MALONATE ION'>MLI</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Classical-complement-pathway_C3/C5_convertase Classical-complement-pathway C3/C5 convertase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.43 3.4.21.43] | ||
| + | |GENE= C2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Complement component C2a''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2I6Q is a [ | + | 2I6Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I6Q OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of complement component C2A: implications for convertase formation and substrate binding., Milder FJ, Raaijmakers HC, Vandeputte MD, Schouten A, Huizinga EG, Romijn RA, Hemrika W, Roos A, Daha MR, Gros P, Structure. 2006 Oct;14(10):1587-97. PMID:[http:// | + | Structure of complement component C2A: implications for convertase formation and substrate binding., Milder FJ, Raaijmakers HC, Vandeputte MD, Schouten A, Huizinga EG, Romijn RA, Hemrika W, Roos A, Daha MR, Gros P, Structure. 2006 Oct;14(10):1587-97. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17027507 17027507] |
[[Category: Classical-complement-pathway C3/C5 convertase]] | [[Category: Classical-complement-pathway C3/C5 convertase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| Line 33: | Line 42: | ||
[[Category: von willebrand factor-a domain]] | [[Category: von willebrand factor-a domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:26:06 2008'' |
Revision as of 15:26, 20 March 2008
| |||||||
| , resolution 2.100Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | C2a (Homo sapiens) | ||||||
| Activity: | Classical-complement-pathway C3/C5 convertase, with EC number 3.4.21.43 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complement component C2a
Contents |
Overview
C2a provides the catalytic center to the convertase complexes of the classical and lectin-binding pathways of complement activation. We determined two crystal structures of full-length C2a, with and without a pseudo ligand bound. Both structures reveal a near-active conformation of the catalytic center of the serine protease domains, while the von Willebrand factor A-type domains display an intermediate activation state of helix alpha7 with an open, activated metal-ion-dependent adhesion site. The open adhesion site likely serves to enhance the affinity for the ligand C4b, similar to "inside-out" signaling in integrins. Surprisingly, the N-terminal residues of C2a are buried in a crevice near helix alpha7, indicative of a structural switch between C2 and C2a. Extended loops on the protease domain possibly envelop the protruding anaphylatoxin domain of the substrate C3. Together with a putative substrate-induced completion of the oxyanion hole, this may contribute to the high substrate specificity of the convertases.
Disease
Known diseases associated with this structure: C2 deficiency OMIM:[217000], Macular degeneration, age-related, reduced risk of OMIM:[217000]
About this Structure
2I6Q is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of complement component C2A: implications for convertase formation and substrate binding., Milder FJ, Raaijmakers HC, Vandeputte MD, Schouten A, Huizinga EG, Romijn RA, Hemrika W, Roos A, Daha MR, Gros P, Structure. 2006 Oct;14(10):1587-97. PMID:17027507
Page seeded by OCA on Thu Mar 20 17:26:06 2008
Categories: Classical-complement-pathway C3/C5 convertase | Homo sapiens | Single protein | Daha, M R. | Gros, P. | Hemrika, W. | Huizinga, E G. | Milder, F J. | Raaijmakers, H C.A. | Romijn, R A. | Roos, A. | Schouten, A. | Vandeputte, D A.A. | MLI | MN | NAG | Serine protease domain | Von willebrand factor-a domain
