2i7z
From Proteopedia
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| - | [[Image:2i7z.gif|left|200px]] | + | [[Image:2i7z.gif|left|200px]] |
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| - | '''GAAA tetraloop receptor complex with associated manganese ions.''' | + | {{Structure |
| + | |PDB= 2i7z |SIZE=350|CAPTION= <scene name='initialview01'>2i7z</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN6:MANGANESE ION, 6 WATERS COORDINATED'>MN6</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''GAAA tetraloop receptor complex with associated manganese ions.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2I7Z is a [ | + | 2I7Z is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I7Z OCA]. |
==Reference== | ==Reference== | ||
| - | Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http:// | + | Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17119098 17119098] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Butcher, S E.]] | [[Category: Butcher, S E.]] | ||
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[[Category: rna tertiary structure]] | [[Category: rna tertiary structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:26:32 2008'' |
Revision as of 15:26, 20 March 2008
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GAAA tetraloop receptor complex with associated manganese ions.
Overview
Metal ions are critical for the proper folding of RNA, and the GAAA tetraloop-receptor is necessary for the optimal folding and function of many RNAs. We have used NMR to investigate the role of metal ions in the structure of the tetraloop-receptor in solution. The NMR data indicate native tertiary structure is formed under a wide range of ionic conditions. The lack of conformational adaptation in response to very different ionic conditions argues against a structural role for divalent ions. Nuclear Overhauser effects to cobalt hexammine and paramagnetic relaxation enhancement induced by manganese ions were used to determine the NMR structures of the tetraloop receptor in association with metal ions, providing the first atomic-level view of these interactions in the solution state. Five manganese and two cobalt hexammine ions could be localized to the RNA surface. The locations of the associated metal ions are similar, but not identical to, those of previously determined crystal structures. The sites of association are in general agreement with nonlinear Poisson-Boltzmann calculations of the electrostatic surface, emphasizing the general importance of diffusely associated ions in RNA tertiary structure.
About this Structure
2I7Z is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Role of metal ions in the tetraloop-receptor complex as analyzed by NMR., Davis JH, Foster TR, Tonelli M, Butcher SE, RNA. 2007 Jan;13(1):76-86. Epub 2006 Nov 21. PMID:17119098
Page seeded by OCA on Thu Mar 20 17:26:32 2008
