2idn
From Proteopedia
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| - | [[Image:2idn.gif|left|200px]] | + | [[Image:2idn.gif|left|200px]] |
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| - | '''NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site''' | + | {{Structure |
| + | |PDB= 2idn |SIZE=350|CAPTION= <scene name='initialview01'>2idn</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IDN is a [ | + | 2IDN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDN OCA]. |
==Reference== | ==Reference== | ||
| - | A new modified thrombin binding aptamer containing a 5'-5' inversion of polarity site., Martino L, Virno A, Randazzo A, Virgilio A, Esposito V, Giancola C, Bucci M, Cirino G, Mayol L, Nucleic Acids Res. 2006;34(22):6653-62. Epub 2006 Dec 1. PMID:[http:// | + | A new modified thrombin binding aptamer containing a 5'-5' inversion of polarity site., Martino L, Virno A, Randazzo A, Virgilio A, Esposito V, Giancola C, Bucci M, Cirino G, Mayol L, Nucleic Acids Res. 2006;34(22):6653-62. Epub 2006 Dec 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17145716 17145716] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Giancola, C.]] | [[Category: Giancola, C.]] | ||
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[[Category: Randazzo, A.]] | [[Category: Randazzo, A.]] | ||
[[Category: Virno, A.]] | [[Category: Virno, A.]] | ||
| - | [[Category: 5'- | + | [[Category: 5'-5s']] |
[[Category: anticoagulant]] | [[Category: anticoagulant]] | ||
[[Category: dna]] | [[Category: dna]] | ||
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[[Category: tba]] | [[Category: tba]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:28:14 2008'' |
Revision as of 15:28, 20 March 2008
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NMR structure of a new modified Thrombin Binding Aptamer containing a 5'-5' inversion of polarity site
Overview
The solution structure of a new modified thrombin binding aptamer (TBA) containing a 5'-5' inversion of polarity site, namely d(3'GGT5'-5'TGGTGTGGTTGG3'), is reported. NMR and CD spectroscopy, as well as molecular dynamic and mechanic calculations, have been used to characterize the 3D structure. The modified oligonucleotide is characterized by a chair-like structure consisting of two G-tetrads connected by three edge-wise TT, TGT and TT loops. d(3'GGT5'-5'TGGTGTGGTTGG3') is characterized by an unusual folding, being three strands parallel to each other and only one strand oriented in opposite manner. This led to an anti-anti-anti-syn and syn-syn-syn-anti arrangement of the Gs in the two tetrads. The thermal stability of the modified oligonucleotide is 4 degrees C higher than the corresponding unmodified TBA. d(3'GGT5'-5'TGGTGTGGTTGG3') continues to display an anticoagulant activity, even if decreased with respect to the TBA.
About this Structure
2IDN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A new modified thrombin binding aptamer containing a 5'-5' inversion of polarity site., Martino L, Virno A, Randazzo A, Virgilio A, Esposito V, Giancola C, Bucci M, Cirino G, Mayol L, Nucleic Acids Res. 2006;34(22):6653-62. Epub 2006 Dec 1. PMID:17145716
Page seeded by OCA on Thu Mar 20 17:28:14 2008
Categories: Protein complex | Giancola, C. | Martino, L. | Mayol, L. | Randazzo, A. | Virno, A. | 5'-5s' | Anticoagulant | Dna | Inversion of polarity | Quadruplex | Tba
