2ido

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[[Image:2ido.gif|left|200px]]<br /><applet load="2ido" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ido.gif|left|200px]]
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caption="2ido, resolution 2.10&Aring;" />
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'''Structure of the E. coli Pol III epsilon-Hot proofreading complex'''<br />
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{{Structure
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|PDB= 2ido |SIZE=350|CAPTION= <scene name='initialview01'>2ido</scene>, resolution 2.10&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TMP:THYMIDINE-5'-PHOSPHATE'>TMP</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7]
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|GENE= dnaQ, mutD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), hot ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10711 Enterobacteria phage P21])
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}}
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'''Structure of the E. coli Pol III epsilon-Hot proofreading complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2IDO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=TMP:'>TMP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDO OCA].
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2IDO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p21 Enterobacteria phage p21] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDO OCA].
==Reference==
==Reference==
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Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex., Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC, J Biol Chem. 2006 Dec 15;281(50):38466-71. Epub 2006 Sep 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16973612 16973612]
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Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex., Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC, J Biol Chem. 2006 Dec 15;281(50):38466-71. Epub 2006 Sep 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16973612 16973612]
[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: Enterobacteria phage p21]]
[[Category: Enterobacteria phage p21]]
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[[Category: polymerase]]
[[Category: polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:51:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:28:15 2008''

Revision as of 15:28, 20 March 2008

Image:2ido.gif


PDB ID 2ido

Drag the structure with the mouse to rotate
, resolution 2.10Å
Ligands: , and
Gene: dnaQ, mutD (Escherichia coli), hot (Enterobacteria phage P21)
Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7
Coordinates: save as pdb, mmCIF, xml



Structure of the E. coli Pol III epsilon-Hot proofreading complex


Overview

The epsilon subunit of Escherichia coli DNA polymerase III possesses 3'-exonucleolytic proofreading activity. Within the Pol III core, epsilon is tightly bound between the alpha subunit (DNA polymerase) and subunit. Here, we present the crystal structure of epsilon in complex with HOT, the bacteriophage P1-encoded homolog of , at 2.1 A resolution. The epsilon-HOT interface is defined by two areas of contact: an interaction of the previously unstructured N terminus of HOT with an edge of the epsilon central beta-sheet as well as interactions between HOT and the catalytically important helix alpha1-loop-helix alpha2 motif of epsilon. This structure provides insight into how HOT and, by implication, may stabilize the epsilon subunit, thus promoting efficient proofreading during chromosomal replication.

About this Structure

2IDO is a Protein complex structure of sequences from Enterobacteria phage p21 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex., Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC, J Biol Chem. 2006 Dec 15;281(50):38466-71. Epub 2006 Sep 13. PMID:16973612

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