2iew
From Proteopedia
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| - | [[Image:2iew.jpg|left|200px]] | + | [[Image:2iew.jpg|left|200px]] |
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| - | '''Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae''' | + | {{Structure |
| + | |PDB= 2iew |SIZE=350|CAPTION= <scene name='initialview01'>2iew</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Inositol-polyphosphate_multikinase Inositol-polyphosphate multikinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.151 2.7.1.151] | ||
| + | |GENE= IPK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IEW is a [ | + | 2IEW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IEW OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity., Holmes W, Jogl G, J Biol Chem. 2006 Dec 8;281(49):38109-16. Epub 2006 Oct 18. PMID:[http:// | + | Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity., Holmes W, Jogl G, J Biol Chem. 2006 Dec 8;281(49):38109-16. Epub 2006 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17050532 17050532] |
[[Category: Inositol-polyphosphate multikinase]] | [[Category: Inositol-polyphosphate multikinase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: atp-grasp fold related]] | [[Category: atp-grasp fold related]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:28:41 2008'' |
Revision as of 15:28, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Gene: | IPK2 (Saccharomyces cerevisiae) | ||||||
| Activity: | Inositol-polyphosphate multikinase, with EC number 2.7.1.151 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Inositol Phosphate Multikinase Ipk2 from S. cerevisiae
Overview
Inositol polyphosphates perform essential functions as second messengers in eukaryotic cells, and their cellular levels are regulated by inositol phosphate kinases. Most of these enzymes belong to the inositol phosphate kinase superfamily, which consists of three subgroups, inositol 3-kinases, inositol phosphate multikinases, and inositol hexakisphosphate kinases. Family members share several strictly conserved signature motifs and are expected to have the same backbone fold, despite very limited overall amino acid sequence identity. Sequence differences are expected to play important roles in defining the different substrate selectivity of these enzymes. To investigate the structural basis for substrate specificity, we have determined the crystal structure of the yeast inositol phosphate multikinase Ipk2 in the apoform and in a complex with ADP and Mn(2+) at up to 2.0A resolution. The overall structure of Ipk2 is related to inositol trisphosphate 3-kinase. The ATP binding site is similar in both enzymes; however, the inositol binding domain is significantly smaller in Ipk2. Replacement of critical side chains in the inositolbinding site suggests how modification of substrate recognition motifs determines enzymatic substrate preference and catalysis.
About this Structure
2IEW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of inositol phosphate multikinase 2 and implications for substrate specificity., Holmes W, Jogl G, J Biol Chem. 2006 Dec 8;281(49):38109-16. Epub 2006 Oct 18. PMID:17050532
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