2ifs
From Proteopedia
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| - | [[Image:2ifs.jpg|left|200px]] | + | [[Image:2ifs.jpg|left|200px]] |
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| - | '''Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide''' | + | {{Structure |
| + | |PDB= 2ifs |SIZE=350|CAPTION= <scene name='initialview01'>2ifs</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= WASPIP, wasl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Homo sapiens, Rattus norvegicus]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IFS is a [ | + | 2IFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_rattus_norvegicus Homo sapiens, rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IFS OCA]. |
==Reference== | ==Reference== | ||
| - | Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP., Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF, J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:[http:// | + | Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP., Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF, J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17229736 17229736] |
[[Category: Homo sapiens, rattus norvegicus]] | [[Category: Homo sapiens, rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: wiskott-aldrich syndrome]] | [[Category: wiskott-aldrich syndrome]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:29:00 2008'' |
Revision as of 15:29, 20 March 2008
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| Gene: | WASPIP, wasl (Homo sapiens, Rattus norvegicus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the N-WASP EVH1 domain in complex with an extended WIP peptide
Overview
The WASP-interacting protein (WIP) targets WASP/WAVE proteins through a constitutive interaction with an amino-terminal enabled/VASP homology (EVH1) domain. Parallel investigations had previously identified two distinct N-WASP binding motifs corresponding to WIP residues 451-461 and 461-485, and we determined the structure of a complex between WIP-(461-485) and the N-WASP EVH1 domain (Volkman, B. F., Prehoda, K. E., Scott, J. A., Peterson, F. C., and Lim, W. A. (2002) Cell 111, 565-576). The present results show that, when combined, the WIP-(451-485) sequence wraps further around the EVH1 domain, extending the interface observed previously. Specific contacts with three WIP epitopes corresponded to regions of high sequence conservation in the verprolin family. A central polyproline motif occupied the canonical binding site but in a reversed orientation relative to other EVH1 complexes. This interaction was augmented in the amino- and carboxyl-terminal directions by additional hydrophobic contacts involving WIP residues 454-459 and 475-478, respectively. Disruption of any of the three WIP epitopes reduced N-WASP binding in cells, demonstrating a functional requirement for the entire binding domain, which is significantly longer than the polyproline motifs recognized by other EVH1 domains.
About this Structure
2IFS is a Single protein structure of sequence from Homo sapiens, rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Multiple WASP-interacting protein recognition motifs are required for a functional interaction with N-WASP., Peterson FC, Deng Q, Zettl M, Prehoda KE, Lim WA, Way M, Volkman BF, J Biol Chem. 2007 Mar 16;282(11):8446-53. Epub 2007 Jan 16. PMID:17229736
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