2igp
From Proteopedia
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| - | [[Image:2igp.jpg|left|200px]] | + | [[Image:2igp.jpg|left|200px]] |
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| - | '''Crystal Structure of Hec1 CH domain''' | + | {{Structure |
| + | |PDB= 2igp |SIZE=350|CAPTION= <scene name='initialview01'>2igp</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= HEC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of Hec1 CH domain''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IGP is a [ | + | 2IGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IGP OCA]. |
==Reference== | ==Reference== | ||
| - | The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment., Wei RR, Al-Bassam J, Harrison SC, Nat Struct Mol Biol. 2007 Jan;14(1):54-9. Epub 2006 Dec 31. PMID:[http:// | + | The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment., Wei RR, Al-Bassam J, Harrison SC, Nat Struct Mol Biol. 2007 Jan;14(1):54-9. Epub 2006 Dec 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17195848 17195848] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Wei, R R.]] | [[Category: Wei, R R.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
| - | [[Category: alpha | + | [[Category: alpha helice]] |
[[Category: calponin homology (ch) domain]] | [[Category: calponin homology (ch) domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:29:20 2008'' |
Revision as of 15:29, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | HEC1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Hec1 CH domain
Overview
Kinetochores are multicomponent assemblies that connect chromosomal centromeres to mitotic-spindle microtubules. The Ndc80 complex is an essential core element of kinetochores, conserved from yeast to humans. It is a rod-like assembly of four proteins- Ndc80p (HEC1 in humans), Nuf2p, Spc24p and Spc25p. We describe here the crystal structure of the most conserved region of HEC1, which lies at one end of the rod and near the N terminus of the polypeptide chain. It folds into a calponin-homology domain, resembling the microtubule-binding domain of the plus-end-associated protein EB1. We show that an Ndc80p-Nuf2p heterodimer binds microtubules in vitro. The less conserved, N-terminal segment of Ndc80p contributes to the interaction and may be a crucial regulatory element. We propose that the Ndc80 complex forms a direct link between kinetochore core components and spindle microtubules.
About this Structure
2IGP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The Ndc80/HEC1 complex is a contact point for kinetochore-microtubule attachment., Wei RR, Al-Bassam J, Harrison SC, Nat Struct Mol Biol. 2007 Jan;14(1):54-9. Epub 2006 Dec 31. PMID:17195848
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