2imt

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[[Image:2imt.gif|left|200px]]<br /><applet load="2imt" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2imt.gif|left|200px]]
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caption="2imt, resolution 1.49&Aring;" />
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'''The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site'''<br />
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{{Structure
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|PDB= 2imt |SIZE=350|CAPTION= <scene name='initialview01'>2imt</scene>, resolution 1.49&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|ACTIVITY=
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|GENE= BAK1, BAK, BCL2L7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2IMT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMT OCA].
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2IMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IMT OCA].
==Reference==
==Reference==
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The X-ray structure of a BAK homodimer reveals an inhibitory zinc binding site., Moldoveanu T, Liu Q, Tocilj A, Watson M, Shore G, Gehring K, Mol Cell. 2006 Dec 8;24(5):677-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17157251 17157251]
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The X-ray structure of a BAK homodimer reveals an inhibitory zinc binding site., Moldoveanu T, Liu Q, Tocilj A, Watson M, Shore G, Gehring K, Mol Cell. 2006 Dec 8;24(5):677-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17157251 17157251]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: dimer]]
[[Category: dimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:54:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:31:15 2008''

Revision as of 15:31, 20 March 2008

Image:2imt.gif


PDB ID 2imt

Drag the structure with the mouse to rotate
, resolution 1.49Å
Ligands:
Gene: BAK1, BAK, BCL2L7 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



The X-ray Structure of a Bak Homodimer Reveals an Inhibitory Zinc Binding Site


Overview

BAK/BAX-mediated mitochondrial outer-membrane permeabilization (MOMP) drives cell death during development and tissue homeostasis from zebrafish to humans. In most cancers, this pathway is inhibited by BCL-2 family antiapoptotic members, which bind and block the action of proapoptotic BCL proteins. We report the 1.5 A crystal structure of calpain-proteolysed BAK, cBAK, to reveal a zinc binding site that regulates its activity via homodimerization. cBAK contains an occluded BH3 peptide binding pocket that binds a BID BH3 peptide only weakly . Nonetheless, cBAK requires activation by truncated BID to induce cytochrome c release in mitochondria isolated from bak/bax double-knockout mouse embryonic fibroblasts. The BAK-mediated MOMP is inhibited by low micromolar zinc levels. This inhibition is alleviated by mutation of the zinc-coordination site in BAK. Our results link directly the antiapoptotic effects of zinc to BAK.

About this Structure

2IMT is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The X-ray structure of a BAK homodimer reveals an inhibitory zinc binding site., Moldoveanu T, Liu Q, Tocilj A, Watson M, Shore G, Gehring K, Mol Cell. 2006 Dec 8;24(5):677-88. PMID:17157251

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