2io5
From Proteopedia
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| - | [[Image:2io5.jpg|left|200px]] | + | [[Image:2io5.jpg|left|200px]] |
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| - | '''Crystal structure of the CIA- histone H3-H4 complex''' | + | {{Structure |
| + | |PDB= 2io5 |SIZE=350|CAPTION= <scene name='initialview01'>2io5</scene>, resolution 2.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= asf1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Histone H3.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Histone H4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the CIA- histone H3-H4 complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IO5 is a [ | + | 2IO5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IO5 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4., Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T, Nature. 2007 Mar 15;446(7133):338-41. Epub 2007 Feb 11. PMID:[http:// | + | Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4., Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T, Nature. 2007 Mar 15;446(7133):338-41. Epub 2007 Feb 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17293877 17293877] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: histone]] | [[Category: histone]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:31:39 2008'' |
Revision as of 15:31, 20 March 2008
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| , resolution 2.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | asf1a (Homo sapiens), Histone H3.1 (Xenopus laevis), Histone H4 (Xenopus laevis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the CIA- histone H3-H4 complex
Overview
CIA (CCG1-interacting factor A)/ASF1, which is the most conserved histone chaperone among the eukaryotes, was genetically identified as a factor for an anti-silencing function (Asf1) by yeast genetic screening. Shortly after that, the CIA-histone-H3-H4 complex was isolated from Drosophila as a histone chaperone CAF-1 stimulator. Human CIA-I/II (ASF1a/b) was identified as a histone chaperone that interacts with the bromodomain-an acetylated-histone-recognizing domain-of CCG1, in the general transcription initiation factor TFIID. Intensive studies have revealed that CIA/ASF1 mediates nucleosome assembly by forming a complex with another histone chaperone in human cells and yeast, and is involved in DNA replication, transcription, DNA repair and silencing/anti-silencing in yeast. CIA/ASF1 was shown as a major storage chaperone for soluble histones in proliferating human cells. Despite all these biochemical and biological functional analyses, the structure-function relationship of the nucleosome assembly/disassembly activity of CIA/ASF1 has remained elusive. Here we report the crystal structure, at 2.7 A resolution, of CIA-I in complex with histones H3 and H4. The structure shows the histone H3-H4 dimer's mutually exclusive interactions with another histone H3-H4 dimer and CIA-I. The carboxy-terminal beta-strand of histone H4 changes its partner from the beta-strand in histone H2A to that of CIA-I through large conformational change. In vitro functional analysis demonstrated that CIA-I has a histone H3-H4 tetramer-disrupting activity. Mutants with weak histone H3-H4 dimer binding activity showed critical functional effects on cellular processes related to transcription. The histone H3-H4 tetramer-disrupting activity of CIA/ASF1 and the crystal structure of the CIA/ASF1-histone-H3-H4 dimer complex should give insights into mechanisms of both nucleosome assembly/disassembly and nucleosome semi-conservative replication.
About this Structure
2IO5 is a Protein complex structure of sequences from Homo sapiens and Xenopus laevis. Full crystallographic information is available from OCA.
Reference
Structure and function of the histone chaperone CIA/ASF1 complexed with histones H3 and H4., Natsume R, Eitoku M, Akai Y, Sano N, Horikoshi M, Senda T, Nature. 2007 Mar 15;446(7133):338-41. Epub 2007 Feb 11. PMID:17293877
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