2iv2

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[[Image:2iv2.gif|left|200px]]<br /><applet load="2iv2" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2iv2.gif|left|200px]]
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caption="2iv2, resolution 2.27&Aring;" />
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'''REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''<br />
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{{Structure
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|PDB= 2iv2 |SIZE=350|CAPTION= <scene name='initialview01'>2iv2</scene>, resolution 2.27&Aring;
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|SITE= <scene name='pdbsite=AC1:S+Binding+Site+For+Chain+X'>AC1</scene>
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|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=2MD:GUANYLATE-O'-PHOSPHORIC+ACID+MONO-(2-AMINO-5,6-DIMERCAPTO-4-OXO-3,5,6,7,8A,9,10,10A-OCTAHYDRO-4H-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-7-YLMETHYL)+ESTER'>2MD</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene> and <scene name='pdbligand=S:SULFUR ATOM'>S</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2]
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|GENE=
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}}
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'''REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2IV2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=2MD:'>2MD</scene>, <scene name='pdbligand=MGD:'>MGD</scene>, <scene name='pdbligand=MO:'>MO</scene> and <scene name='pdbligand=S:'>S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Formate_dehydrogenase Formate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.2 1.2.1.2] Known structural/functional Site: <scene name='pdbsite=AC1:S+Binding+Site+For+Chain+X'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IV2 OCA].
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2IV2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IV2 OCA].
==Reference==
==Reference==
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Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism., Raaijmakers HC, Romao MJ, J Biol Inorg Chem. 2006 Oct;11(7):849-54. Epub 2006 Jul 8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16830149 16830149]
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Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism., Raaijmakers HC, Romao MJ, J Biol Inorg Chem. 2006 Oct;11(7):849-54. Epub 2006 Jul 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16830149 16830149]
[[Category: Formate dehydrogenase]]
[[Category: Formate dehydrogenase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: S]]
[[Category: S]]
[[Category: SF4]]
[[Category: SF4]]
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[[Category: 4fe-4s]]
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[[Category: 4fe-4]]
[[Category: anaerobic]]
[[Category: anaerobic]]
[[Category: complete proteome]]
[[Category: complete proteome]]
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[[Category: nad]]
[[Category: nad]]
[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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[[Category: secys]]
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[[Category: secy]]
[[Category: selenium]]
[[Category: selenium]]
[[Category: selenocysteine]]
[[Category: selenocysteine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:56:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:33:45 2008''

Revision as of 15:33, 20 March 2008

Image:2iv2.gif


PDB ID 2iv2

Drag the structure with the mouse to rotate
, resolution 2.27Å
Sites:
Ligands: , , , and
Activity: Formate dehydrogenase, with EC number 1.2.1.2
Coordinates: save as pdb, mmCIF, xml



REINTERPRETATION OF REDUCED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI


Overview

Re-evaluation of the crystallographic data of the molybdenum-containing E. coli formate dehydrogenase H (Boyington et al. Science 275:1305-1308, 1997), reported in two redox states, reveals important structural differences for the formate-reduced form, with large implications for the reaction mechanism proposed in that work. We have re-refined the reduced structure with revised protocols and found substantial rearrangement in some parts of it. The original model is essentially correct but an important loop close to the molybdenum active site was mistraced, and, therefore, catalytic relevant residues were located in wrong positions. In particular selenocysteine-140, a ligand of molybdenum in the original work, and essential for catalysis, is no longer bound to the metal after reduction of the enzyme with formate. These results are incompatible with the originally proposed reaction mechanism. On the basis of our new interpretation, we have revised and proposed a new reaction mechanism, which reconciles the new X-ray model with previous biochemical and extended X-ray absorption fine structure data.

About this Structure

2IV2 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Formate-reduced E. coli formate dehydrogenase H: The reinterpretation of the crystal structure suggests a new reaction mechanism., Raaijmakers HC, Romao MJ, J Biol Inorg Chem. 2006 Oct;11(7):849-54. Epub 2006 Jul 8. PMID:16830149

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