2ixs
From Proteopedia
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| - | [[Image:2ixs.gif|left|200px]] | + | [[Image:2ixs.gif|left|200px]] |
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| - | '''STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE''' | + | {{Structure |
| + | |PDB= 2ixs |SIZE=350|CAPTION= <scene name='initialview01'>2ixs</scene>, resolution 2.00Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Trs+Binding+Site+For+Chain+B'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene> and <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2IXS is a [ | + | 2IXS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_diastaticus Streptomyces diastaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IXS OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:[http:// | + | The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16962970 16962970] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces diastaticus]] | [[Category: Streptomyces diastaticus]] | ||
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[[Category: sdai]] | [[Category: sdai]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:34:46 2008'' |
Revision as of 15:34, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE
Overview
Rare-cutting restriction enzymes are important tools in genome analysis. We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the cleavage site). Unlike orthodox Type IIP enzymes, which are single domain proteins, the SdaI monomer is composed of two structural domains. The N domain contains a classical winged helix-turn-helix (wHTH) DNA binding motif, while the C domain shows a typical restriction endonuclease fold. The active site of SdaI is located within the C domain and represents a variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence specificity are clustered on the recognition helix of the wHTH motif at the N domain. The modular architecture of SdaI, wherein one domain mediates DNA binding while the other domain is predicted to catalyze hydrolysis, distinguishes SdaI from previously characterized restriction enzymes interacting with symmetric recognition sequences.
About this Structure
2IXS is a Single protein structure of sequence from Streptomyces diastaticus. Full crystallographic information is available from OCA.
Reference
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970
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