2j19

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[[Image:2j19.jpg|left|200px]]<br /><applet load="2j19" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2j19.jpg|left|200px]]
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caption="2j19, resolution 1.750&Aring;" />
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'''FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)'''<br />
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{{Structure
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|PDB= 2j19 |SIZE=350|CAPTION= <scene name='initialview01'>2j19</scene>, resolution 1.750&Aring;
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|SITE= <scene name='pdbsite=AC1:Br+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10]
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|GENE=
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}}
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'''FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2J19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=AC1:Br+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J19 OCA].
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2J19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leptoxyphium_fumago Leptoxyphium fumago]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J19 OCA].
==Reference==
==Reference==
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Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17211068 17211068]
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Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17211068 17211068]
[[Category: Chloride peroxidase]]
[[Category: Chloride peroxidase]]
[[Category: Leptoxyphium fumago]]
[[Category: Leptoxyphium fumago]]
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[[Category: pyrrolidone carboxylic acid]]
[[Category: pyrrolidone carboxylic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:58:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:36:09 2008''

Revision as of 15:36, 20 March 2008

Image:2j19.jpg


PDB ID 2j19

Drag the structure with the mouse to rotate
, resolution 1.750Å
Sites:
Ligands: , , , and
Activity: Chloride peroxidase, with EC number 1.11.1.10
Coordinates: save as pdb, mmCIF, xml



FERROUS CHLOROPEROXIDASE (HIGH DOSE DATA SET)


Overview

The X-ray crystallographic analysis of redox-active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X-ray beam may be exploited to generate otherwise short-lived reaction intermediates of metalloproteins, it is generally an undesired feature. Here, the X-ray-induced reduction of the three heme proteins myoglobin, cytochrome P450cam and chloroperoxidase has been followed by on-line UV-Vis absorption spectroscopy. All three systems showed a very rapid reduction of the heme iron. In chloroperoxidase the change of the ionization state from ferric to ferrous heme is associated with a movement of the heme-coordinating water molecule. The influence of the energy of the incident X-ray photons and of the presence of scavengers on the apparent reduction rate of ferric myoglobin crystals was analyzed.

About this Structure

2J19 is a Single protein structure of sequence from Leptoxyphium fumago. Full crystallographic information is available from OCA.

Reference

Cryoradiolytic reduction of crystalline heme proteins: analysis by UV-Vis spectroscopy and X-ray crystallography., Beitlich T, Kuhnel K, Schulze-Briese C, Shoeman RL, Schlichting I, J Synchrotron Radiat. 2007 Jan;14(Pt 1):11-23. Epub 2006 Dec 15. PMID:17211068

Page seeded by OCA on Thu Mar 20 17:36:09 2008

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