2j59
From Proteopedia
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| - | [[Image:2j59.gif|left|200px]] | + | [[Image:2j59.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX''' | + | {{Structure |
| + | |PDB= 2j59 |SIZE=350|CAPTION= <scene name='initialview01'>2j59</scene>, resolution 2.1Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Gtp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Gtp+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Gtp+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+C'>AC6</scene>, <scene name='pdbsite=AC7:Gtp+Binding+Site+For+Chain+D'>AC7</scene>, <scene name='pdbsite=AC8:Mg+Binding+Site+For+Chain+D'>AC8</scene>, <scene name='pdbsite=AC9:Gtp+Binding+Site+For+Chain+E'>AC9</scene>, <scene name='pdbsite=BC1:Mg+Binding+Site+For+Chain+E'>BC1</scene>, <scene name='pdbsite=BC2:Gtp+Binding+Site+For+Chain+F'>BC2</scene>, <scene name='pdbsite=BC3:Mg+Binding+Site+For+Chain+F'>BC3</scene>, <scene name='pdbsite=BC4:So4+Binding+Site+For+Chain+M'>BC4</scene>, <scene name='pdbsite=BC5:So4+Binding+Site+For+Chain+N'>BC5</scene>, <scene name='pdbsite=BC6:So4+Binding+Site+For+Chain+O'>BC6</scene>, <scene name='pdbsite=BC7:So4+Binding+Site+For+Chain+P'>BC7</scene>, <scene name='pdbsite=BC8:So4+Binding+Site+For+Chain+Q'>BC8</scene>, <scene name='pdbsite=BC9:So4+Binding+Site+For+Chain+R'>BC9</scene>, <scene name='pdbsite=CC1:Dio+Binding+Site+For+Chain+A'>CC1</scene>, <scene name='pdbsite=CC2:Dio+Binding+Site+For+Chain+B'>CC2</scene>, <scene name='pdbsite=CC3:Dio+Binding+Site+For+Chain+F'>CC3</scene>, <scene name='pdbsite=CC4:Edo+Binding+Site+For+Chain+C'>CC4</scene>, <scene name='pdbsite=CC5:Edo+Binding+Site+For+Chain+O'>CC5</scene> and <scene name='pdbsite=CC6:Edo+Binding+Site+For+Chain+E'>CC6</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J59 is a [ | + | 2J59 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J59 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes., Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A, EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:[http:// | + | Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes., Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A, EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17347647 17347647] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
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[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:37:24 2008'' |
Revision as of 15:37, 20 March 2008
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| , resolution 2.1Å | |||||||
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| Sites: | , , , , , , , , , , , , , , , , , , , , , , and | ||||||
| Ligands: | , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE ARF1:ARHGAP21-ARFBD COMPLEX
Overview
ARHGAP21 is a Rho family GTPase-activating protein (RhoGAP) that controls the Arp2/3 complex and F-actin dynamics at the Golgi complex by regulating the activity of the small GTPase Cdc42. ARHGAP21 is recruited to the Golgi by binding to another small GTPase, ARF1. Here, we present the crystal structure of the activated GTP-bound form of ARF1 in a complex with the Arf-binding domain (ArfBD) of ARHGAP21 at 2.1 A resolution. We show that ArfBD comprises a PH domain adjoining a C-terminal alpha helix, and that ARF1 interacts with both of these structural motifs through its switch regions and triggers structural rearrangement of the PH domain. We used site-directed mutagenesis to confirm that both the PH domain and the helical motif are essential for the binding of ArfBD to ARF1 and for its recruitment to the Golgi. Our data demonstrate that two well-known small GTPase-binding motifs, the PH domain and the alpha helical motif, can combine to create a novel mode of binding to Arfs.
About this Structure
2J59 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for ARF1-mediated recruitment of ARHGAP21 to Golgi membranes., Menetrey J, Perderiset M, Cicolari J, Dubois T, Elkhatib N, El Khadali F, Franco M, Chavrier P, Houdusse A, EMBO J. 2007 Apr 4;26(7):1953-62. Epub 2007 Mar 8. PMID:17347647
Page seeded by OCA on Thu Mar 20 17:37:24 2008
Categories: Homo sapiens | Mus musculus | Protein complex | Chavrier, P. | Cicolari, J. | Dubois, T. | Franco, M. | Houdusse, A. | Khadali, F El. | Khatib, N El. | Menetrey, J. | Perderiset, M. | DIO | EDO | GTP | MG | SO4 | Actin organization | Arf | Arf1 | Arfbd | Arhgap21 | Golgi apparatus | Hydrolase | Myristate | Nucleotide-binding | Protein transport | Rhogap protein | Small gtp-binding protein | Transport
