2j6a
From Proteopedia
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| - | [[Image:2j6a.jpg|left|200px]] | + | [[Image:2j6a.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.''' | + | {{Structure |
| + | |PDB= 2j6a |SIZE=350|CAPTION= <scene name='initialview01'>2j6a</scene>, resolution 1.70Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2J6A is a [ | + | 2J6A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J6A OCA]. |
==Reference== | ==Reference== | ||
| - | The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:[http:// | + | The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17008308 17008308] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: translation termination]] | [[Category: translation termination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:37:48 2008'' |
Revision as of 15:37, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF S. CEREVISIAE YNR046W, A ZINC FINGER PROTEIN FROM THE ERF1 METHYLTRANSFERASE COMPLEX.
Overview
Protein release factor eRF1 in Saccharomyces cerevisiae, in complex with eRF3 and GTP, is methylated on a functionally crucial Gln residue by the S-adenosylmethionine-dependent methyltransferase Ydr140w. Here we show that eRF1 methylation, in addition to these previously characterized components, requires a 15-kDa zinc-binding protein, Ynr046w. Co-expression in Escherichia coli of Ynr046w and Ydr140w allows the latter to be recovered in soluble form rather than as inclusion bodies, and the two proteins co-purify on nickel-nitrilotriacetic acid chromatography when Ydr140w alone carries a His tag. The crystal structure of Ynr046w has been determined to 1.7 A resolution. It comprises a zinc-binding domain built from both the N- and C-terminal sequences and an inserted domain, absent from bacterial and archaeal orthologs of the protein, composed of three alpha-helices. The active methyltransferase is the heterodimer Ydr140w.Ynr046w, but when alone, both in solution and in crystals, Ynr046w appears to be a homodimer. The Ynr046w eRF1 methyltransferase subunit is shared by the tRNA methyltransferase Trm11p and probably by two other enzymes containing a Rossman fold.
About this Structure
2J6A is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast., Heurgue-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH, J Biol Chem. 2006 Nov 24;281(47):36140-8. Epub 2006 Sep 28. PMID:17008308
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