2jhn

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[[Image:2jhn.gif|left|200px]]<br /><applet load="2jhn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2jhn.gif|left|200px]]
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caption="2jhn, resolution 1.80&Aring;" />
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'''3-METHYLADENINE DNA-GLYCOSYLASE FROM ARCHAEOGLOBUS FULGIDUS'''<br />
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{{Structure
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|PDB= 2jhn |SIZE=350|CAPTION= <scene name='initialview01'>2jhn</scene>, resolution 1.80&Aring;
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|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=MBO:MERCURIBENZOIC+ACID'>MBO</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21]
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|GENE=
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}}
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'''3-METHYLADENINE DNA-GLYCOSYLASE FROM ARCHAEOGLOBUS FULGIDUS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2JHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with <scene name='pdbligand=HG:'>HG</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MBO:'>MBO</scene>, <scene name='pdbligand=MES:'>MES</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JHN OCA].
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2JHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JHN OCA].
==Reference==
==Reference==
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Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA., Leiros I, Nabong MP, Grosvik K, Ringvoll J, Haugland GT, Uldal L, Reite K, Olsbu IK, Knaevelsrud I, Moe E, Andersen OA, Birkeland NK, Ruoff P, Klungland A, Bjelland S, EMBO J. 2007 Apr 18;26(8):2206-17. Epub 2007 Mar 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17396151 17396151]
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Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA., Leiros I, Nabong MP, Grosvik K, Ringvoll J, Haugland GT, Uldal L, Reite K, Olsbu IK, Knaevelsrud I, Moe E, Andersen OA, Birkeland NK, Ruoff P, Klungland A, Bjelland S, EMBO J. 2007 Apr 18;26(8):2206-17. Epub 2007 Mar 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17396151 17396151]
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: DNA-3-methyladenine glycosylase II]]
[[Category: DNA-3-methyladenine glycosylase II]]
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[[Category: dna repair]]
[[Category: dna repair]]
[[Category: hydrolase]]
[[Category: hydrolase]]
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[[Category: hyperthermophiles]]
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[[Category: hyperthermophile]]
[[Category: n1-methyladenine]]
[[Category: n1-methyladenine]]
[[Category: n3-methylcytosine]]
[[Category: n3-methylcytosine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:03:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:42:01 2008''

Revision as of 15:42, 20 March 2008

Image:2jhn.gif


PDB ID 2jhn

Drag the structure with the mouse to rotate
, resolution 1.80Å
Sites:
Ligands: , , , and
Activity: DNA-3-methyladenine glycosylase II, with EC number 3.2.2.21
Coordinates: save as pdb, mmCIF, xml



3-METHYLADENINE DNA-GLYCOSYLASE FROM ARCHAEOGLOBUS FULGIDUS


Overview

N(1)-methyladenine (m(1)A) and N(3)-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m(1)A and m(3)C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1,N(6)-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity.

About this Structure

2JHN is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Structural basis for enzymatic excision of N1-methyladenine and N3-methylcytosine from DNA., Leiros I, Nabong MP, Grosvik K, Ringvoll J, Haugland GT, Uldal L, Reite K, Olsbu IK, Knaevelsrud I, Moe E, Andersen OA, Birkeland NK, Ruoff P, Klungland A, Bjelland S, EMBO J. 2007 Apr 18;26(8):2206-17. Epub 2007 Mar 29. PMID:17396151

Page seeded by OCA on Thu Mar 20 17:42:01 2008

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