2js2
From Proteopedia
(New page: 200px<br /><applet load="2js2" size="350" color="white" frame="true" align="right" spinBox="true" caption="2js2" /> '''Solution structure of first SH3 domain of ad...) |
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| - | [[Image:2js2.jpg|left|200px]] | + | [[Image:2js2.jpg|left|200px]] |
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| - | '''Solution structure of first SH3 domain of adaptor Nck''' | + | {{Structure |
| + | |PDB= 2js2 |SIZE=350|CAPTION= <scene name='initialview01'>2js2</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= NCK1, NCK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of first SH3 domain of adaptor Nck''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JS2 is a [ | + | 2JS2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JS2 OCA]. |
==Reference== | ==Reference== | ||
| - | Specificity Determinants of a Novel Nck Interaction with the Juxtamembrane Domain of the Epidermal Growth Factor Receptor(,)., Hake MJ, Choowongkomon K, Kostenko O, Carlin CR, Sonnichsen FD, Biochemistry. 2008 Feb 13;. PMID:[http:// | + | Specificity Determinants of a Novel Nck Interaction with the Juxtamembrane Domain of the Epidermal Growth Factor Receptor(,)., Hake MJ, Choowongkomon K, Kostenko O, Carlin CR, Sonnichsen FD, Biochemistry. 2008 Feb 13;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18269246 18269246] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: signaling protein]] | [[Category: signaling protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:44:44 2008'' |
Revision as of 15:44, 20 March 2008
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| Gene: | NCK1, NCK (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of first SH3 domain of adaptor Nck
Overview
Nck is a ubiquitously expressed adaptor protein containing Src homology 2 (SH2) and Src homology 3 (SH3) domains. It integrates downstream effector proteins with cell membrane receptors, such as the epidermal growth factor receptor (EGFR). EGFR plays a critical role in cellular proliferation and differentiation. The 45-residue juxtamembrane domain of EGFR (JM), located between the transmembrane and kinase domains, regulates receptor activation and trafficking to the basolateral membrane of polarized epithelia through a proline-rich motif that resembles a consensus SH3 domain binding site. We demonstrate here that the JM region can bind to Nck, showing a notable binding preference for the second SH3 domain. To elucidate the structural determinants for this interaction, we have determined the NMR solution structures of both the first and second Nck SH3 domains (Nck1-1 and Nck1-2). These domains adopt a canonical SH3 beta-barrel-like fold, containing five antiparallel strands separated by three loop regions and one 3 10-helical turn. Chemical shift perturbation studies have identified the residues that form the binding cleft of Nck1-2, which are primarily located in the RT and n-Src loops. JM binds to Nck1-2 with an affinity of approximately 80 microM through a positively charged sequence near the N-terminus, as opposed to the polyproline sequence. The two Nck SH3 domains exhibit both steric and electrostatic differences in their RT-Src and n-Src loops, and a model of the Nck1-2 domain complexed with the JM highlights the factors that define the putative binding mode for this ligand.
About this Structure
2JS2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Specificity Determinants of a Novel Nck Interaction with the Juxtamembrane Domain of the Epidermal Growth Factor Receptor(,)., Hake MJ, Choowongkomon K, Kostenko O, Carlin CR, Sonnichsen FD, Biochemistry. 2008 Feb 13;. PMID:18269246
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