2jug
From Proteopedia
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| - | [[Image:2jug.jpg|left|200px]] | + | [[Image:2jug.jpg|left|200px]] |
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| - | '''Multienzyme Docking in Hybrid Megasynthetases''' | + | {{Structure |
| + | |PDB= 2jug |SIZE=350|CAPTION= <scene name='initialview01'>2jug</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= tubC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=38 Angiococcus disciformis]) | ||
| + | }} | ||
| + | |||
| + | '''Multienzyme Docking in Hybrid Megasynthetases''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JUG is a [ | + | 2JUG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Angiococcus_disciformis Angiococcus disciformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JUG OCA]. |
==Reference== | ==Reference== | ||
| - | Multienzyme docking in hybrid megasynthetases., Richter CD, Nietlispach D, Broadhurst RW, Weissman KJ, Nat Chem Biol. 2008 Jan;4(1):75-81. Epub 2007 Dec 9. PMID:[http:// | + | Multienzyme docking in hybrid megasynthetases., Richter CD, Nietlispach D, Broadhurst RW, Weissman KJ, Nat Chem Biol. 2008 Jan;4(1):75-81. Epub 2007 Dec 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18066054 18066054] |
[[Category: Angiococcus disciformis]] | [[Category: Angiococcus disciformis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: tubulysin]] | [[Category: tubulysin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:45:11 2008'' |
Revision as of 15:45, 20 March 2008
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| Gene: | tubC (Angiococcus disciformis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Multienzyme Docking in Hybrid Megasynthetases
Overview
Hybrid multienzyme systems composed of polyketide synthase (PKS) and nonribosomal polypeptide synthetase (NRPS) modules direct the biosynthesis of clinically valuable natural products in bacteria. The fidelity of this process depends on specific recognition between successive polypeptides in each assembly line-interactions that are mediated by terminal 'docking domains'. We have identified a new family of N-terminal docking domains, exemplified by TubCdd from the tubulysin system of Angiococcus disciformis An d48. TubCdd is homodimeric, which suggests that NRPS subunits in mixed systems self-associate to interact with partner PKS homodimers. The NMR structure of TubCdd reveals a new fold featuring an exposed beta-hairpin that serves as the binding site for the C-terminal docking domain of the partner polypeptide. The pattern of charged residues on the contact surface of the beta-hairpin is a key determinant of the interaction and seems to constitute a 'docking code' that can be used to alter binding affinity.
About this Structure
2JUG is a Single protein structure of sequence from Angiococcus disciformis. Full crystallographic information is available from OCA.
Reference
Multienzyme docking in hybrid megasynthetases., Richter CD, Nietlispach D, Broadhurst RW, Weissman KJ, Nat Chem Biol. 2008 Jan;4(1):75-81. Epub 2007 Dec 9. PMID:18066054
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