2jv1
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2jv1.jpg|left|200px]] | + | [[Image:2jv1.jpg|left|200px]] |
| - | + | ||
| - | '''NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures''' | + | {{Structure |
| + | |PDB= 2jv1 |SIZE=350|CAPTION= <scene name='initialview01'>2jv1</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= INS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2JV1 is a [ | + | 2JV1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JV1 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of human insulin monomer in water/acetonitrile solution., Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L, J Biomol NMR. 2008 Jan;40(1):55-64. Epub 2007 Nov 27. PMID:[http:// | + | Structure of human insulin monomer in water/acetonitrile solution., Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L, J Biomol NMR. 2008 Jan;40(1):55-64. Epub 2007 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18040865 18040865] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 17: | Line 26: | ||
[[Category: 35% cd3cn]] | [[Category: 35% cd3cn]] | ||
[[Category: carbohydrate metabolism]] | [[Category: carbohydrate metabolism]] | ||
| - | [[Category: cleavage on pair of basic | + | [[Category: cleavage on pair of basic residue]] |
[[Category: diabetes mellitus]] | [[Category: diabetes mellitus]] | ||
[[Category: disease mutation]] | [[Category: disease mutation]] | ||
| Line 28: | Line 37: | ||
[[Category: secreted]] | [[Category: secreted]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:45:17 2008'' |
Revision as of 15:45, 20 March 2008
| |||||||
| Gene: | INS (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of human insulin monomer in 35% CD3CN zinc free, 50 structures
Overview
Here we present evidence that in water/acetonitrile solvent detailed structural and dynamic information can be obtained for important proteins that are naturally present as oligomers under native conditions. An NMR-derived human insulin monomer structure in H(2)O/CD(3)CN, 65/35 vol%, pH 3.6 is presented and compared with the available X-ray structure of a monomer that forms part of a hexamer (Acta Crystallogr. 2003 Sec. D59, 474) and with NMR structures in water and organic cosolvent. Detailed analysis using PFGSE NMR, temperature-dependent NMR, dilution experiments and CSI proves that the structure is monomeric in the concentration and temperature ranges 0.1-3 mM and 10-30 degrees C, respectively. The presence of long-range interstrand NOEs, as found in the crystal structure of the monomer, provides the evidence for conservation of the tertiary structure. Starting from structures calculated by the program CYANA, two different molecular dynamics simulated annealing refinement protocols were applied, either using the program AMBER in vacuum (AMBER_VC), or including a generalized Born solvent model (AMBER_GB).
About this Structure
2JV1 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of human insulin monomer in water/acetonitrile solution., Bocian W, Sitkowski J, Bednarek E, Tarnowska A, Kawecki R, Kozerski L, J Biomol NMR. 2008 Jan;40(1):55-64. Epub 2007 Nov 27. PMID:18040865
Page seeded by OCA on Thu Mar 20 17:45:17 2008
