2jvb
From Proteopedia
(New page: 200px<br /><applet load="2jvb" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jvb" /> '''Solution Structure of Catalytic Domain of yD...) |
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| - | [[Image:2jvb.jpg|left|200px]] | + | [[Image:2jvb.jpg|left|200px]] |
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| - | '''Solution Structure of Catalytic Domain of yDcp2''' | + | {{Structure |
| + | |PDB= 2jvb |SIZE=350|CAPTION= <scene name='initialview01'>2jvb</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= DCP2, PSU1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
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| + | '''Solution Structure of Catalytic Domain of yDcp2''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2JVB is a [ | + | 2JVB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JVB OCA]. |
==Reference== | ==Reference== | ||
| - | mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2., Deshmukh MV, Jones BN, Quang-Dang DU, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD, Mol Cell. 2008 Feb 15;29(3):324-36. PMID:[http:// | + | mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2., Deshmukh MV, Jones BN, Quang-Dang DU, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD, Mol Cell. 2008 Feb 15;29(3):324-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18280238 18280238] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rna-binding]] | [[Category: rna-binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:45:22 2008'' |
Revision as of 15:45, 20 March 2008
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| Gene: | DCP2, PSU1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Catalytic Domain of yDcp2
Overview
Cap hydrolysis by Dcp2 is a critical step in several eukaryotic mRNA decay pathways. Processing requires access to cap-proximal nucleotides and the coordinated assembly of a decapping mRNP, but the mechanism of substrate recognition and regulation by protein interactions have remained elusive. Using NMR spectroscopy and kinetic analyses, we show that yeast Dcp2 resolves interactions with the cap and RNA body using a bipartite surface that forms a channel intersecting the catalytic and regulatory Dcp1-binding domains. The interaction with cap is weak but specific and requires binding of the RNA body to a dynamic interface. The catalytic step is stimulated by Dcp1 and its interaction domain, likely through a substrate-induced conformational change. Thus, activation of the decapping mRNP is restricted by access to 5'-proximal nucleotides, a feature that could act as a checkpoint in mRNA metabolism.
About this Structure
2JVB is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
mRNA Decapping Is Promoted by an RNA-Binding Channel in Dcp2., Deshmukh MV, Jones BN, Quang-Dang DU, Flinders J, Floor SN, Kim C, Jemielity J, Kalek M, Darzynkiewicz E, Gross JD, Mol Cell. 2008 Feb 15;29(3):324-36. PMID:18280238
Page seeded by OCA on Thu Mar 20 17:45:22 2008
