2ltn

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[[Image:2ltn.jpg|left|200px]]<br /><applet load="2ltn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ltn.jpg|left|200px]]
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caption="2ltn, resolution 1.7&Aring;" />
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'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''<br />
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{{Structure
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|PDB= 2ltn |SIZE=350|CAPTION= <scene name='initialview01'>2ltn</scene>, resolution 1.7&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2LTN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTN OCA].
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2LTN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pisum_sativum Pisum sativum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LTN OCA].
==Reference==
==Reference==
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Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2708344 2708344]
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Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2708344 2708344]
[[Category: Pisum sativum]]
[[Category: Pisum sativum]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: lectin]]
[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:07:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:46:51 2008''

Revision as of 15:46, 20 March 2008

Image:2ltn.jpg


PDB ID 2ltn

Drag the structure with the mouse to rotate
, resolution 1.7Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



DESIGN, EXPRESSION, AND CRYSTALLIZATION OF RECOMBINANT LECTIN FROM THE GARDEN PEA (PISUM SATIVUM)


Overview

The propeptide form of the lectin from the garden pea (Pisum sativum agglutinin) has been expressed in Escherichia coli by attaching its cDNA to an inducible promoter. By a number of criteria, including the ability to form dimers, hemagglutination titer, Western blot, and enzyme-linked immunosorbent assay, the resulting propeptide molecule is virtually indistinguishable from the mature proteolytically processed lectin isolated from peas. Preliminary crystallization experiments using the recombinant propeptide lectin yield crystals in space group P2(1)2(1)2(1) with a = 64.8 A, b = 73.8 A, and c = 109.0 A (1 A = 0.1 nm) that diffract to 2.8-A resolution. This unit cell size is quite similar to the unit cell determined for native pea lectin, suggesting that the overall structure of the recombinant prolectin is virtually identical.

About this Structure

2LTN is a Protein complex structure of sequences from Pisum sativum. Full crystallographic information is available from OCA.

Reference

Design, expression, and crystallization of recombinant lectin from the garden pea (Pisum sativum)., Prasthofer T, Phillips SR, Suddath FL, Engler JA, J Biol Chem. 1989 Apr 25;264(12):6793-6. PMID:2708344

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