2ngr
From Proteopedia
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| - | [[Image:2ngr.jpg|left|200px]] | + | [[Image:2ngr.jpg|left|200px]] |
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| - | '''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.''' | + | {{Structure |
| + | |PDB= 2ngr |SIZE=350|CAPTION= <scene name='initialview01'>2ngr</scene>, resolution 1.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene> and <scene name='pdbligand=AF3:ALUMINUM FLUORIDE'>AF3</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NGR is a [ | + | 2NGR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NGR OCA]. |
==Reference== | ==Reference== | ||
| - | Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:[http:// | + | Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9846874 9846874] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transition state]] | [[Category: transition state]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:47:49 2008'' |
Revision as of 15:47, 20 March 2008
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| , resolution 1.9Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRANSITION STATE COMPLEX FOR GTP HYDROLYSIS BY CDC42: COMPARISONS OF THE HIGH RESOLUTION STRUCTURES FOR CDC42 BOUND TO THE ACTIVE AND CATALYTICALLY COMPROMISED FORMS OF THE CDC42-GAP.
Overview
The Rho-related small GTP-binding protein Cdc42 has a low intrinsic GTPase activity that is significantly enhanced by its specific GTPase-activating protein, Cdc42GAP. In this report, we present the tertiary structure for the aluminum fluoride-promoted complex between Cdc42 and a catalytically active domain of Cdc42GAP as well as the complex between Cdc42 and the catalytically compromised Cdc42GAP(R305A) mutant. These structures, which mimic the transition state for the GTP hydrolytic reaction, show the presence of an AIF3 molecule, as was seen for the corresponding Ras-p120RasGAP complex, but in contrast to what has been reported for the Rho-Cdc42GAP complex or for heterotrimeric G protein alpha subunits, where AIF4- was observed. The Cdc42GAP stabilizes both the switch I and switch II domains of Cdc42 and contributes a highly conserved arginine (Arg 305) to the active site. Comparison of the structures for the wild type and mutant Cdc42GAP complexes provides important insights into the GAP-catalyzed GTP hydrolytic reaction.
About this Structure
2NGR is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structures of Cdc42 bound to the active and catalytically compromised forms of Cdc42GAP., Nassar N, Hoffman GR, Manor D, Clardy JC, Cerione RA, Nat Struct Biol. 1998 Dec;5(12):1047-52. PMID:9846874
Page seeded by OCA on Thu Mar 20 17:47:49 2008
Categories: Homo sapiens | Protein complex | Cerione, R. | Clardy, J. | Hoffman, G. | Nassar, N. | AF3 | GDP | MG | Alf3. | Cdc42 | G-protein | Gap | Transition state
