2nmv
From Proteopedia
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| - | [[Image:2nmv.gif|left|200px]] | + | [[Image:2nmv.gif|left|200px]] |
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| - | '''Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA''' | + | {{Structure |
| + | |PDB= 2nmv |SIZE=350|CAPTION= <scene name='initialview01'>2nmv</scene>, resolution 2.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=NML:N-METHYLACETAMIDE'>NML</scene> and <scene name='pdbligand=FLU:2-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-BENZOIC ACID'>FLU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= uvrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
| + | }} | ||
| + | |||
| + | '''Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NMV is a [ | + | 2NMV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NMV OCA]. |
==Reference== | ==Reference== | ||
| - | Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:[http:// | + | Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17097086 17097086] |
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: t-fluorescein]] | [[Category: t-fluorescein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:48:19 2008'' |
Revision as of 15:48, 20 March 2008
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| , resolution 2.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Gene: | uvrB (Bacillus subtilis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA
Overview
UvrB is the damage recognition element of the highly conserved UvrABC pathway that functions in the removal of bulky DNA adducts. Pivotal to this is the formation of a damage detection complex that relies on the ability of UvrB to locate and sequester diverse lesions. Whilst structures of UvrB bound to DNA have recently been reported, none address the issue of lesion recognition. Here, we describe the crystal structure of UvrB bound to a pentanucleotide containing a single fluorescein-adducted thymine that reveals a unique mechanism for damage detection entirely dependent on the exclusion of lesions larger than an undamaged nucleotide.
About this Structure
2NMV is a Protein complex structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Damage detection by the UvrABC pathway: crystal structure of UvrB bound to fluorescein-adducted DNA., Waters TR, Eryilmaz J, Geddes S, Barrett TE, FEBS Lett. 2006 Nov 27;580(27):6423-7. Epub 2006 Nov 3. PMID:17097086
Page seeded by OCA on Thu Mar 20 17:48:19 2008
Categories: Bacillus subtilis | Protein complex | Barrett, T E. | Eryilmaz, J. | Geddes, S. | Waters, T R. | ADP | FLU | NML | Hairpin | Protein-dna complex | T-fluorescein
