2nnf
From Proteopedia
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| - | [[Image:2nnf.jpg|left|200px]] | + | [[Image:2nnf.jpg|left|200px]] |
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| - | '''Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum''' | + | {{Structure |
| + | |PDB= 2nnf |SIZE=350|CAPTION= <scene name='initialview01'>2nnf</scene>, resolution 2.390Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NNF is a [ | + | 2NNF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlorobium_limicola Chlorobium limicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NNF OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray crystallographic analysis of the sulfur carrier protein SoxY from Chlorobium limicola f. thiosulfatophilum reveals a tetrameric structure., Stout J, Van Driessche G, Savvides SN, Van Beeumen J, Protein Sci. 2007 Apr;16(4):589-601. Epub 2007 Feb 27. PMID:[http:// | + | X-ray crystallographic analysis of the sulfur carrier protein SoxY from Chlorobium limicola f. thiosulfatophilum reveals a tetrameric structure., Stout J, Van Driessche G, Savvides SN, Van Beeumen J, Protein Sci. 2007 Apr;16(4):589-601. Epub 2007 Feb 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17327392 17327392] |
[[Category: Chlorobium limicola]] | [[Category: Chlorobium limicola]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sulfur binding protein]] | [[Category: sulfur binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:48:34 2008'' |
Revision as of 15:48, 20 March 2008
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| , resolution 2.390Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the sulfur carrier protein SoxY from Chlorobium limicola f thiosulfatophilum
Overview
Dissimilatory oxidation of thiosulfate in the green sulfur bacterium Chlorobium limicola f. thiosulfatophilum is carried out by the ubiquitous sulfur-oxidizing (Sox) multi-enzyme system. In this system, SoxY plays a key role, functioning as the sulfur substrate-binding protein that offers its sulfur substrate, which is covalently bound to a conserved C-terminal cysteine, to another oxidizing Sox enzyme. Here, we report the crystal structures of a stand-alone SoxY protein of C. limicola f. thiosulfatophilum, solved at 2.15 A and 2.40 A resolution using X-ray diffraction data collected at 100 K and room temperature, respectively. The structure reveals a monomeric Ig-like protein, with an N-terminal alpha-helix, that oligomerizes into a tetramer via conserved contact regions between the monomers. The tetramer can be described as a dimer of dimers that exhibits one large hydrophobic contact region in each dimer and two small hydrophilic interface patches in the tetramer. At the tetramer interface patch, two conserved redox-active C-terminal cysteines form an intersubunit disulfide bridge. Intriguingly, SoxY exhibits a dimer/tetramer equilibrium that is dependent on the redox state of the cysteines and on the type of sulfur substrate component bound to them. Taken together, the dimer/tetramer equilibrium, the specific interactions between the subunits in the tetramer, and the significant conservation level of the interfaces strongly indicate that these SoxY oligomers are biologically relevant.
About this Structure
2NNF is a Single protein structure of sequence from Chlorobium limicola. Full crystallographic information is available from OCA.
Reference
X-ray crystallographic analysis of the sulfur carrier protein SoxY from Chlorobium limicola f. thiosulfatophilum reveals a tetrameric structure., Stout J, Van Driessche G, Savvides SN, Van Beeumen J, Protein Sci. 2007 Apr;16(4):589-601. Epub 2007 Feb 27. PMID:17327392
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