2npi
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2npi.gif|left|200px]] | + | [[Image:2npi.gif|left|200px]] |
| - | + | ||
| - | '''Clp1-ATP-Pcf11 complex''' | + | {{Structure |
| + | |PDB= 2npi |SIZE=350|CAPTION= <scene name='initialview01'>2npi</scene>, resolution 2.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CLP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), PCF11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Clp1-ATP-Pcf11 complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2NPI is a [ | + | 2NPI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPI OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor., Noble CG, Beuth B, Taylor IA, Nucleic Acids Res. 2007;35(1):87-99. Epub 2006 Dec 6. PMID:[http:// | + | Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor., Noble CG, Beuth B, Taylor IA, Nucleic Acids Res. 2007;35(1):87-99. Epub 2006 Dec 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17151076 17151076] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| Line 22: | Line 31: | ||
[[Category: ternary complex]] | [[Category: ternary complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:49:23 2008'' |
Revision as of 15:49, 20 March 2008
| |||||||
| , resolution 2.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | CLP1 (Saccharomyces cerevisiae), PCF11 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Clp1-ATP-Pcf11 complex
Overview
Pcf11 and Clp1 are subunits of cleavage factor IA (CFIA), an essential polyadenylation factor in Saccahromyces cerevisiae. We have determined the structure of a ternary complex of Clp1 together with ATP and the Clp1-binding region of Pcf11. Clp1 contains three domains, a small N-terminal beta sandwich domain, a C-terminal domain containing a novel alpha/beta-fold and a central domain that binds ATP. The arrangement of the nucleotide binding site is similar to that observed in SIMIBI-class ATPase subunits found in other multisubunit macromolecular complexes. However, despite this similarity, nucleotide hydrolysis does not occur. The Pcf11 binding site is also located in the central domain where three highly conserved residues in Pcf11 mediate many of the protein-protein interactions. We propose that this conserved Clp1-Pcf11 interaction is responsible for maintaining a tight coupling between the Clp1 nucleotide binding subunit and the other components of the polyadenylation machinery. Moreover, we suggest that this complex represents a stabilized ATP bound form of Clp1 that requires the participation of other non-CFIA processing factors in order to initiate timely ATP hydrolysis during 3' end processing.
About this Structure
2NPI is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a nucleotide-bound Clp1-Pcf11 polyadenylation factor., Noble CG, Beuth B, Taylor IA, Nucleic Acids Res. 2007;35(1):87-99. Epub 2006 Dec 6. PMID:17151076
Page seeded by OCA on Thu Mar 20 17:49:23 2008
