2nqv

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[[Image:2nqv.gif|left|200px]]<br /><applet load="2nqv" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2nqv.gif|left|200px]]
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caption="2nqv, resolution 2.820&Aring;" />
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'''MoeA D228A'''<br />
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{{Structure
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|PDB= 2nqv |SIZE=350|CAPTION= <scene name='initialview01'>2nqv</scene>, resolution 2.820&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY=
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|GENE= moeA, bisB, chlE, narE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''MoeA D228A'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2NQV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NQV OCA].
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2NQV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NQV OCA].
==Reference==
==Reference==
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Mutational analysis of Escherichia coli MoeA: two functional activities map to the active site cleft., Nichols JD, Xiang S, Schindelin H, Rajagopalan KV, Biochemistry. 2007 Jan 9;46(1):78-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17198377 17198377]
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Mutational analysis of Escherichia coli MoeA: two functional activities map to the active site cleft., Nichols JD, Xiang S, Schindelin H, Rajagopalan KV, Biochemistry. 2007 Jan 9;46(1):78-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17198377 17198377]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: mpt]]
[[Category: mpt]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:09:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:49:54 2008''

Revision as of 15:49, 20 March 2008

Image:2nqv.gif


PDB ID 2nqv

Drag the structure with the mouse to rotate
, resolution 2.820Å
Ligands:
Gene: moeA, bisB, chlE, narE (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



MoeA D228A


Overview

The molybdenum cofactor is ubiquitous in nature, and the pathway for Moco biosynthesis is conserved in all three domains of life. Recent work has helped to illuminate one of the most enigmatic steps in Moco biosynthesis, ligation of metal to molybdopterin (the organic component of the cofactor) to form the active cofactor. In Escherichia coli, the MoeA protein mediates ligation of Mo to molybdopterin while the MogA protein enhances this process in an ATP-dependent manner. The X-ray crystal structures for both proteins have been previously described as well as two essential MogA residues, Asp49 and Asp82. Here we describe a detailed mutational analysis of the MoeA protein. Variants of conserved residues at the putative active site of MoeA were analyzed for a loss of function in two different, previously described assays, one employing moeA- crude extracts and the other utilizing a defined system. Oddly, no correlation was observed between the activity in the two assays. In fact, our results showed a general trend toward an inverse relationship between the activity in each assay. Moco binding studies indicated a strong correlation between a variant's ability to bind Moco and its activity in the purified component assay. Crystal structures of the functionally characterized MoeA variants revealed no major structural changes, indicating that the functional differences observed are not due to disruption of the protein structure. On the basis of these results, two different functional areas were assigned to regions at or near the MoeA active site cleft.

About this Structure

2NQV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Mutational analysis of Escherichia coli MoeA: two functional activities map to the active site cleft., Nichols JD, Xiang S, Schindelin H, Rajagopalan KV, Biochemistry. 2007 Jan 9;46(1):78-86. PMID:17198377

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