2kiu

From Proteopedia

(Difference between revisions)

Revision as of 13:57, 19 January 2015

Solution structure and backbone dynamics of the DNA-binding domain of FOXP1: Insight into its domain swapping

Structural highlights

2kiu is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	3p14.1, FOXP1, HSPC215 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Disease

[FOXP1_HUMAN] MALT lymphoma;Precursor B-cell acute lymphoblastic leukemia;Intellectual disability-severe speech delay-mild dysmorphism syndrome. A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5. The disease is caused by mutations affecting the gene represented in this entry.^[1]

Function

[FOXP1_HUMAN] Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B-cell development (By similarity).^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

FOXP1 belongs to the P-subfamily of forkhead transcription factors and contains a conserved forkhead DNA-binding domain. According to size exclusion chromatography analysis, the forkhead domain of FOXP1 existed as a mixture of monomer and dimer. The dissociation constants of the forkhead domain of wild-type, C61S, and C61Y mutants of FOXP1 were 27.3, 28.8, and 332.0 muM, respectively. In contrast, FOXP1 A39P mutant formed only a monomer. NMR analysis also showed that FOXP1 C61S and C61Y mutants existed as a mixture. The solution structure of FOXP1 A39P/C61Y mutant was similar to the X-ray structure of the FOXP2 monomer. Comparison of backbone dynamics of FOXP1 A39P/C61Y and C61Y mutants showed that the residues preceding helix 3, the hinge region, exhibited the largest conformational exchange in FOXP1 monomer. The A39 residue of FOXP1 dimer has a lower order parameter with internal motion on the ps-ns timescale, suggesting that the dynamics of the hinge region of FOXP1 are important in the formation of the swapped dimer. The analysis also showed that the residues exhibiting the motions on the ps-ns and mus-ms timescales were located at the DNA-binding surface of FOXP1, suggesting the interactions between FOXP1 and DNA may be highly dynamic.

Solution structure and backbone dynamics of the DNA-binding domain of FOXP1: Insight into its domain swapping and DNA binding.,Chu YP, Chang CH, Shiu JH, Chang YT, Chen CY, Chuang WJ Protein Sci. 2011 May;20(5):908-24. doi: 10.1002/pro.626. Epub 2011 Apr, 11. PMID:21416545^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hamdan FF, Daoud H, Rochefort D, Piton A, Gauthier J, Langlois M, Foomani G, Dobrzeniecka S, Krebs MO, Joober R, Lafreniere RG, Lacaille JC, Mottron L, Drapeau P, Beauchamp MH, Phillips MS, Fombonne E, Rouleau GA, Michaud JL. De novo mutations in FOXP1 in cases with intellectual disability, autism, and language impairment. Am J Hum Genet. 2010 Nov 12;87(5):671-8. doi: 10.1016/j.ajhg.2010.09.017. Epub, 2010 Oct 14. PMID:20950788 doi:http://dx.doi.org/10.1016/j.ajhg.2010.09.017
↑ Hamdan FF, Daoud H, Rochefort D, Piton A, Gauthier J, Langlois M, Foomani G, Dobrzeniecka S, Krebs MO, Joober R, Lafreniere RG, Lacaille JC, Mottron L, Drapeau P, Beauchamp MH, Phillips MS, Fombonne E, Rouleau GA, Michaud JL. De novo mutations in FOXP1 in cases with intellectual disability, autism, and language impairment. Am J Hum Genet. 2010 Nov 12;87(5):671-8. doi: 10.1016/j.ajhg.2010.09.017. Epub, 2010 Oct 14. PMID:20950788 doi:http://dx.doi.org/10.1016/j.ajhg.2010.09.017
↑ Chu YP, Chang CH, Shiu JH, Chang YT, Chen CY, Chuang WJ. Solution structure and backbone dynamics of the DNA-binding domain of FOXP1: Insight into its domain swapping and DNA binding. Protein Sci. 2011 May;20(5):908-24. doi: 10.1002/pro.626. Epub 2011 Apr, 11. PMID:21416545 doi:10.1002/pro.626

1 Structural highlights
2 Disease
3 Function
4 Evolutionary Conservation
5 Publication Abstract from PubMed
6 See Also
7 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2kiu"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2kiu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KIU FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">3p14.1, FOXP1, HSPC215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">3p14.1, FOXP1, HSPC215 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kiu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kiu RCSB], [http://www.ebi.ac.uk/pdbsum/2kiu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kiu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kiu RCSB], [http://www.ebi.ac.uk/pdbsum/2kiu PDBsum]</span></td></tr>
-<table>
+</table>
 == Disease ==
 [[http://www.uniprot.org/uniprot/FOXP1_HUMAN FOXP1_HUMAN]] MALT lymphoma;Precursor B-cell acute lymphoblastic leukemia;Intellectual disability-severe speech delay-mild dysmorphism syndrome. A chromosomal aberration involving FOXP1 is found in acute lymphoblastic leukemia. Translocation t(9;3)(p13;p14.1) with PAX5.  The disease is caused by mutations affecting the gene represented in this entry.<ref>PMID:20950788</ref>
@@ Line 36: / Line 36: @@
 </StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Chu, Y.]]
+[[Category: Chu, Y]]
-[[Category: Chuang, W.]]
+[[Category: Chuang, W]]
 [[Category: Dna binding protein]]
 [[Category: Dna-binding]]

2kiu

From Proteopedia

Revision as of 13:57, 19 January 2015

Solution structure and backbone dynamics of the DNA-binding domain of FOXP1: Insight into its domain swapping

Structural highlights

Disease

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox