2ns6
From Proteopedia
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| - | [[Image:2ns6.jpg|left|200px]] | + | [[Image:2ns6.jpg|left|200px]] |
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| - | '''Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162''' | + | {{Structure |
| + | |PDB= 2ns6 |SIZE=350|CAPTION= <scene name='initialview01'>2ns6</scene>, resolution 2.100Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= mobA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NS6 is a [ | + | 2NS6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NS6 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the minimal relaxase domain of MobA at 2.1 A resolution., Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD, J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:[http:// | + | The structure of the minimal relaxase domain of MobA at 2.1 A resolution., Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD, J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17157875 17157875] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
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[[Category: nickase]] | [[Category: nickase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:50:22 2008'' |
Revision as of 15:50, 20 March 2008
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| , resolution 2.100Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | mobA (Pseudomonas aeruginosa) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162
Overview
The plasmid R1162 encodes proteins that enable its conjugative mobilization between bacterial cells. It can transfer between many different species and is one of the most promiscuous of the mobilizable plasmids. The plasmid-encoded protein MobA, which has both nicking and priming activities on single-stranded DNA, is essential for mobilization. The nicking, or relaxase, activity has been localized to the 186 residue N-terminal domain, called minMobA. We present here the 2.1 A X-ray structure of minMobA. The fold is similar to that seen for two other relaxases, TraI and TrwC. The similarity in fold, and action, suggests these enzymes are evolutionary homologs, despite the lack of any significant amino acid similarity. MinMobA has a well- defined target DNA called oriT. The active site metal is observed near Tyr25, which is known to form a phosphotyrosine adduct with the substrate. A model of the oriT substrate complexed with minMobA has been made, based on observed substrate binding to TrwC and TraI. The model is consistent with observations of substrate base specificity, and provides a rationalization for elements of the likely enzyme mechanism.
About this Structure
2NS6 is a Protein complex structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
The structure of the minimal relaxase domain of MobA at 2.1 A resolution., Monzingo AF, Ozburn A, Xia S, Meyer RJ, Robertus JD, J Mol Biol. 2007 Feb 9;366(1):165-78. Epub 2006 Nov 11. PMID:17157875
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