2ntc
From Proteopedia
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| - | [[Image:2ntc.gif|left|200px]] | + | [[Image:2ntc.gif|left|200px]] |
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| - | '''Crystal Structure of sv40 large T antigen origin binding domain with DNA''' | + | {{Structure |
| + | |PDB= 2ntc |SIZE=350|CAPTION= <scene name='initialview01'>2ntc</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian Virus 40]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of sv40 large T antigen origin binding domain with DNA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NTC is a [ | + | 2NTC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTC OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA., Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A, PLoS Biol. 2007 Feb;5(2):e23. PMID:[http:// | + | The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA., Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A, PLoS Biol. 2007 Feb;5(2):e23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17253903 17253903] |
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: origin binding domain; protein-dna complex; replication]] | [[Category: origin binding domain; protein-dna complex; replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:50:52 2008'' |
Revision as of 15:50, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | large T antigen (Simian Virus 40) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of sv40 large T antigen origin binding domain with DNA
Overview
DNA replication is initiated upon binding of "initiators" to origins of replication. In simian virus 40 (SV40), the core origin contains four pentanucleotide binding sites organized as pairs of inverted repeats. Here we describe the crystal structures of the origin binding domain (obd) of the SV40 large T-antigen (T-ag) both with and without a subfragment of origin-containing DNA. In the co-structure, two T-ag obds are oriented in a head-to-head fashion on the same face of the DNA, and each T-ag obd engages the major groove. Although the obds are very close to each other when bound to this DNA target, they do not contact one another. These data provide a high-resolution structural model that explains site-specific binding to the origin and suggests how these interactions help direct the oligomerization events that culminate in assembly of the helicase-active dodecameric complex of T-ag.
About this Structure
2NTC is a Single protein structure of sequence from Simian virus 40. Full crystallographic information is available from OCA.
Reference
The crystal structure of the SV40 T-antigen origin binding domain in complex with DNA., Meinke G, Phelan P, Moine S, Bochkareva E, Bochkarev A, Bullock PA, Bohm A, PLoS Biol. 2007 Feb;5(2):e23. PMID:17253903
Page seeded by OCA on Thu Mar 20 17:50:52 2008
