2nug
From Proteopedia
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| - | [[Image:2nug.gif|left|200px]] | + | [[Image:2nug.gif|left|200px]] |
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| - | '''Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution''' | + | {{Structure |
| + | |PDB= 2nug |SIZE=350|CAPTION= <scene name='initialview01'>2nug</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | ||
| + | |GENE= rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NUG is a [ | + | 2NUG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NUG OCA]. |
==Reference== | ==Reference== | ||
| - | A stepwise model for double-stranded RNA processing by ribonuclease III., Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X, Mol Microbiol. 2007 Dec 7;67(1):143-154. Epub 2007 Nov 27. PMID:[http:// | + | A stepwise model for double-stranded RNA processing by ribonuclease III., Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X, Mol Microbiol. 2007 Dec 7;67(1):143-154. Epub 2007 Nov 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18047582 18047582] |
[[Category: Aquifex aeolicus]] | [[Category: Aquifex aeolicus]] | ||
[[Category: Ribonuclease III]] | [[Category: Ribonuclease III]] | ||
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[[Category: rna interference]] | [[Category: rna interference]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:51:19 2008'' |
Revision as of 15:51, 20 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | rnc (Aquifex aeolicus) | ||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution
Overview
RNA interference is mediated by small interfering RNAs produced by members of the ribonuclease III (RNase III) family represented by bacterial RNase III and eukaryotic Rnt1p, Drosha and Dicer. For mechanistic studies, bacterial RNase III has been a valuable model system for the family. Previously, we have shown that RNase III uses two catalytic sites to create the 2-nucleotide (nt) 3' overhangs in its products. Here, we present three crystal structures of RNase III in complex with double-stranded RNA, demonstrating how Mg(2+) is essential for the formation of a catalytically competent protein-RNA complex, how the use of two Mg(2+) ions can drive the hydrolysis of each phosphodiester bond, and how conformational changes in both the substrate and the protein are critical elements for assembling the catalytic complex. Moreover, we have modelled a protein-substrate complex and a protein-reaction intermediate (transition state) complex on the basis of the crystal structures. Together, the crystal structures and the models suggest a stepwise mechanism for RNase III to execute the phosphoryl transfer reaction.
About this Structure
2NUG is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
Reference
A stepwise model for double-stranded RNA processing by ribonuclease III., Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X, Mol Microbiol. 2007 Dec 7;67(1):143-154. Epub 2007 Nov 27. PMID:18047582
Page seeded by OCA on Thu Mar 20 17:51:19 2008
