2iy3
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of the E. Coli Signal Regognition Particle== | |
| - | + | <StructureSection load='2iy3' size='340' side='right' caption='[[2iy3]], [[Resolution|resolution]] 16.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[2iy3]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IY3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2IY3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iy3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2iy3 RCSB], [http://www.ebi.ac.uk/pdbsum/2iy3 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iy/2iy3_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The prokaryotic signal recognition particle (SRP) targets membrane proteins into the inner membrane. It binds translating ribosomes and screens the emerging nascent chain for a hydrophobic signal sequence, such as the transmembrane helix of inner membrane proteins. If such a sequence emerges, the SRP binds tightly, allowing the SRP receptor to lock on. This assembly delivers the ribosome-nascent chain complex to the protein translocation machinery in the membrane. Using cryo-electron microscopy and single-particle reconstruction, we obtained a 16 A structure of the Escherichia coli SRP in complex with a translating E. coli ribosome containing a nascent chain with a transmembrane helix anchor. We also obtained structural information on the SRP bound to an empty E. coli ribosome. The latter might share characteristics with a scanning SRP complex, whereas the former represents the next step: the targeting complex ready for receptor binding. High-resolution structures of the bacterial ribosome and of the bacterial SRP components are available, and their fitting explains our electron microscopic density. The structures reveal the regions that are involved in complex formation, provide insight into the conformation of the SRP on the ribosome and indicate the conformational changes that accompany high-affinity SRP binding to ribosome nascent chain complexes upon recognition of the signal sequence. | ||
| - | + | Structure of the E. coli signal recognition particle bound to a translating ribosome.,Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N Nature. 2006 Nov 23;444(7118):503-6. Epub 2006 Oct 29. PMID:17086205<ref>PMID:17086205</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Signal recognition particle protein|Signal recognition particle protein]] | *[[Signal recognition particle protein|Signal recognition particle protein]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Atcc 25104]] | [[Category: Atcc 25104]] | ||
| - | [[Category: Abrahams, J P | + | [[Category: Abrahams, J P]] |
| - | [[Category: Ban, N | + | [[Category: Ban, N]] |
| - | [[Category: Berger, I | + | [[Category: Berger, I]] |
| - | [[Category: Ishikawa, T | + | [[Category: Ishikawa, T]] |
| - | [[Category: Koerten, H K | + | [[Category: Koerten, H K]] |
| - | [[Category: Koning, R I | + | [[Category: Koning, R I]] |
| - | [[Category: Oswald, M | + | [[Category: Oswald, M]] |
| - | [[Category: Schaffitzel, C | + | [[Category: Schaffitzel, C]] |
[[Category: Rna-binding]] | [[Category: Rna-binding]] | ||
[[Category: Rna-binding protein complex]] | [[Category: Rna-binding protein complex]] | ||
[[Category: Signal recognition particle]] | [[Category: Signal recognition particle]] | ||
Revision as of 15:12, 19 January 2015
Structure of the E. Coli Signal Regognition Particle
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