2nxx
From Proteopedia
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| - | [[Image:2nxx.jpg|left|200px]] | + | [[Image:2nxx.jpg|left|200px]] |
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| - | '''Crystal Structure of the Ligand-Binding Domains of the T.castaneum (Coleoptera) Heterodimer EcrUSP Bound to Ponasterone A''' | + | {{Structure |
| + | |PDB= 2nxx |SIZE=350|CAPTION= <scene name='initialview01'>2nxx</scene>, resolution 2.750Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=P1A:2,3,14,20,22-PENTAHYDROXYCHOLEST-7-EN-6-ONE'>P1A</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Ultraspiracle (USP) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]), Ecdysone Receptor (EcR) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Tribolium castaneum]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Ligand-Binding Domains of the T.castaneum (Coleoptera) Heterodimer EcrUSP Bound to Ponasterone A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2NXX is a [ | + | 2NXX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Tribolium_castaneum Tribolium castaneum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NXX OCA]. |
==Reference== | ==Reference== | ||
| - | Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor., Iwema T, Billas IM, Beck Y, Bonneton F, Nierengarten H, Chaumot A, Richards G, Laudet V, Moras D, EMBO J. 2007 Aug 22;26(16):3770-82. Epub 2007 Aug 2. PMID:[http:// | + | Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor., Iwema T, Billas IM, Beck Y, Bonneton F, Nierengarten H, Chaumot A, Richards G, Laudet V, Moras D, EMBO J. 2007 Aug 22;26(16):3770-82. Epub 2007 Aug 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17673910 17673910] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Tribolium castaneum]] | [[Category: Tribolium castaneum]] | ||
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[[Category: hormone/growth factor complex]] | [[Category: hormone/growth factor complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:52:40 2008'' |
Revision as of 15:52, 20 March 2008
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| , resolution 2.750Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Ultraspiracle (USP) (Tribolium castaneum), Ecdysone Receptor (EcR) (Tribolium castaneum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Ligand-Binding Domains of the T.castaneum (Coleoptera) Heterodimer EcrUSP Bound to Ponasterone A
Overview
Retinoid X receptor (RXR) and Ultraspiracle (USP) play a central role as ubiquitous heterodimerization partners of many nuclear receptors. While it has long been accepted that a wide range of ligands can activate vertebrate/mollusc RXRs, the existence and necessity of specific endogenous ligands activating RXR-USP in vivo is still matter of intense debate. Here we report the existence of a novel type of RXR-USP with a ligand-independent functional conformation. Our studies involved Tribolium USP (TcUSP) as representative of most arthropod RXR-USPs, with high sequence homology to vertebrate/mollusc RXRs. The crystal structure of the ligand-binding domain of TcUSP was solved in the context of the functional heterodimer with the ecdysone receptor (EcR). While EcR exhibits a canonical ligand-bound conformation, USP adopts an original apo structure. Our functional data demonstrate that TcUSP is a constitutively silent partner of EcR, and that none of the RXR ligands can bind and activate TcUSP. These findings together with a phylogenetic analysis suggest that RXR-USPs have undergone remarkable functional shifts during evolution and give insight into receptor-ligand binding evolution and dynamics.
About this Structure
2NXX is a Protein complex structure of sequences from Tribolium castaneum. Full crystallographic information is available from OCA.
Reference
Structural and functional characterization of a novel type of ligand-independent RXR-USP receptor., Iwema T, Billas IM, Beck Y, Bonneton F, Nierengarten H, Chaumot A, Richards G, Laudet V, Moras D, EMBO J. 2007 Aug 22;26(16):3770-82. Epub 2007 Aug 2. PMID:17673910
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