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2o0t

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[[Image:2o0t.gif|left|200px]]<br /><applet load="2o0t" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2o0t.gif|left|200px]]
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caption="2o0t, resolution 2.33&Aring;" />
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'''The three dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation'''<br />
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{{Structure
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|PDB= 2o0t |SIZE=350|CAPTION= <scene name='initialview01'>2o0t</scene>, resolution 2.33&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20]
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|GENE= lysA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])
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}}
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'''The three dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2O0T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Diaminopimelate_decarboxylase Diaminopimelate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.20 4.1.1.20] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0T OCA].
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2O0T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O0T OCA].
==Reference==
==Reference==
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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis., Kefala G, Perry LJ, Weiss MS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt, 8):782-4. Epub 2005 Jul 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511157 16511157]
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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis., Kefala G, Perry LJ, Weiss MS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt, 8):782-4. Epub 2005 Jul 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511157 16511157]
[[Category: Diaminopimelate decarboxylase]]
[[Category: Diaminopimelate decarboxylase]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: lysine biosynthesis]]
[[Category: lysine biosynthesis]]
[[Category: plp binding enzyme]]
[[Category: plp binding enzyme]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: tb structural genomics consortium]]
[[Category: tb structural genomics consortium]]
[[Category: tbsgc]]
[[Category: tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:13:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:53:40 2008''

Revision as of 15:53, 20 March 2008

Image:2o0t.gif


PDB ID 2o0t

Drag the structure with the mouse to rotate
, resolution 2.33Å
Ligands:
Gene: lysA (Mycobacterium tuberculosis)
Activity: Diaminopimelate decarboxylase, with EC number 4.1.1.20
Coordinates: save as pdb, mmCIF, xml



The three dimensional structure of diaminopimelate decarboxylase from Mycobacterium tuberculosis reveals a tetrameric enzyme organisation


Overview

Diaminopimelate decarboxylase from Mycobacterium tuberculosis (LysA, DAPDC, Rv1293) has been cloned and heterologously expressed in Escherichia coli, purified using standard chromatographic techniques and crystallized. Preliminary diffraction data analysis suggests the presence of a homotetramer in the asymmetric unit.

About this Structure

2O0T is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of LysA (Rv1293) from Mycobacterium tuberculosis., Kefala G, Perry LJ, Weiss MS, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Aug 1;61(Pt, 8):782-4. Epub 2005 Jul 30. PMID:16511157

Page seeded by OCA on Thu Mar 20 17:53:40 2008

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