1gz5

From Proteopedia

Revision as of 14:55, 29 October 2007

TREHALOSE-6-PHOSPHATE SYNTHASE. OTSA

Overview

Trehalose is a nonreducing disaccharide that plays a major role in many, organisms, most notably in survival and stress responses. In Mycobacterium, tuberculosis, it plays a central role as the carbohydrate core of numerous, immunogenic glycolipids including "cord factor" (trehalose, 6,6'-dimycolate). The classical pathway for trehalose synthesis involves, the condensation of UDP-glucose and glucose-6-phosphate to afford, trehalose-6-phosphate, catalyzed by the retaining glycosyltransferase, OtsA. The configurations of two anomeric positions are set simultaneously, resulting in the formation of a double glycoside. The three-dimensional, structure of the Escherichia coli OtsA, in complex with both UDP and, glucose-6-phosphate, reveals the active site at the interface of two, ... [(full description)]

About this Structure

1GZ5 is a [Single protein] structure of sequence from [Escherichia coli] with G6P, UDP and IMD as [ligands]. Active as [[1]], with EC number [2.4.1.15]. Full crystallographic information is available from [OCA].

Reference

Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA., Gibson RP, Turkenburg JP, Charnock SJ, Lloyd R, Davies GJ, Chem Biol. 2002 Dec;9(12):1337-46. PMID:12498887

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