2oa0
From Proteopedia
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| - | [[Image:2oa0.gif|left|200px]] | + | [[Image:2oa0.gif|left|200px]] |
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| - | '''Crystal structure of Calcium ATPase with bound ADP and cyclopiazonic acid''' | + | {{Structure |
| + | |PDB= 2oa0 |SIZE=350|CAPTION= <scene name='initialview01'>2oa0</scene>, resolution 3.400Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=CZA:(6AR,11AS,11BR)-10-ACETYL-9-HYDROXY-7,7-DIMETHYL-2,6,6A,7,11A,11B-HEXAHYDRO-11H-PYRROLO[1',2':2,3]ISOINDOLO[4,5,6-CD]INDOL-11-ONE'>CZA</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Calcium-transporting_ATPase Calcium-transporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.8 3.6.3.8] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Calcium ATPase with bound ADP and cyclopiazonic acid''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OA0 is a [ | + | 2OA0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA0 OCA]. |
==Reference== | ==Reference== | ||
| - | The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump., Moncoq K, Trieber CA, Young HS, J Biol Chem. 2007 Mar 30;282(13):9748-57. Epub 2007 Jan 26. PMID:[http:// | + | The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump., Moncoq K, Trieber CA, Young HS, J Biol Chem. 2007 Mar 30;282(13):9748-57. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259168 17259168] |
[[Category: Calcium-transporting ATPase]] | [[Category: Calcium-transporting ATPase]] | ||
[[Category: Oryctolagus cuniculus]] | [[Category: Oryctolagus cuniculus]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:57:00 2008'' |
Revision as of 15:57, 20 March 2008
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| , resolution 3.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Calcium-transporting ATPase, with EC number 3.6.3.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Calcium ATPase with bound ADP and cyclopiazonic acid
Overview
The sarcoplasmic reticulum Ca(2+)-ATPase is essential for calcium reuptake in the muscle contraction-relaxation cycle. Here we present structures of a calcium-free state with bound cyclopiazonic acid (CPA) and magnesium fluoride at 2.65 A resolution and a calcium-free state with bound CPA and ADP at 3.4A resolution. In both structures, CPA occupies the calcium access channel delimited by transmembrane segments M1-M4. Inhibition of Ca(2+)-ATPase is stabilized by a polar pocket that surrounds the tetramic acid of CPA and a hydrophobic platform that cradles the inhibitor. The calcium pump residues involved include Gln(56), Leu(61), Val(62), and Asn(101). We conclude that CPA inhibits the calcium pump by blocking the calcium access channel and immobilizing a subset of transmembrane helices. In the E2(CPA) structure, ADP is bound in a distinct orientation within the nucleotide binding pocket. The adenine ring is sandwiched between Arg(489) of the nucleotide-binding domain and Arg(678) of the phosphorylation domain. This mode of binding conforms to an adenine recognition motif commonly found in ATP-dependent proteins.
About this Structure
2OA0 is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump., Moncoq K, Trieber CA, Young HS, J Biol Chem. 2007 Mar 30;282(13):9748-57. Epub 2007 Jan 26. PMID:17259168
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