2oaj
From Proteopedia
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| - | [[Image:2oaj.jpg|left|200px]] | + | [[Image:2oaj.jpg|left|200px]] |
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| - | '''Crystal structure of Sro7 from S. cerevisiae''' | + | {{Structure |
| + | |PDB= 2oaj |SIZE=350|CAPTION= <scene name='initialview01'>2oaj</scene>, resolution 2.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= SNI1, SRO7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of Sro7 from S. cerevisiae''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OAJ is a [ | + | 2OAJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAJ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism., Hattendorf DA, Andreeva A, Gangar A, Brennwald PJ, Weis WI, Nature. 2007 Mar 29;446(7135):567-71. PMID:[http:// | + | Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism., Hattendorf DA, Andreeva A, Gangar A, Brennwald PJ, Weis WI, Nature. 2007 Mar 29;446(7135):567-71. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17392788 17392788] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: wd40 repeat]] | [[Category: wd40 repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:57:14 2008'' |
Revision as of 15:57, 20 March 2008
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| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | SNI1, SRO7 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Sro7 from S. cerevisiae
Overview
Polarized exocytosis requires coordination between the actin cytoskeleton and the exocytic machinery responsible for fusion of secretory vesicles at specific sites on the plasma membrane. Fusion requires formation of a complex between a vesicle-bound R-SNARE and plasma membrane Qa, Qb and Qc SNARE proteins. Proteins in the lethal giant larvae protein family, including lethal giant larvae and tomosyn in metazoans and Sro7 in yeast, interact with Q-SNAREs and are emerging as key regulators of polarized exocytosis. The crystal structure of Sro7 reveals two seven-bladed WD40 beta-propellers followed by a 60-residue-long 'tail', which is bound to the surface of the amino-terminal propeller. Deletion of the Sro7 tail enables binding to the Qbc SNARE region of Sec9 and this interaction inhibits SNARE complex assembly. The N-terminal domain of Sec9 provides a second, high-affinity Sro7 interaction that is unaffected by the tail. The results suggest that Sro7 acts as an allosteric regulator of exocytosis through interactions with factors that control the tail. Sequence alignments indicate that lethal giant larvae and tomosyn have a two-beta-propeller fold similar to that of Sro7, but only tomosyn appears to retain the regulatory tail.
About this Structure
2OAJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism., Hattendorf DA, Andreeva A, Gangar A, Brennwald PJ, Weis WI, Nature. 2007 Mar 29;446(7135):567-71. PMID:17392788
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