2ob1
From Proteopedia
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| - | [[Image:2ob1.gif|left|200px]] | + | [[Image:2ob1.gif|left|200px]] |
| - | + | ||
| - | '''ppm1 with 1,8-ANS''' | + | {{Structure |
| + | |PDB= 2ob1 |SIZE=350|CAPTION= <scene name='initialview01'>2ob1</scene>, resolution 1.900Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= PPM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''ppm1 with 1,8-ANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OB1 is a [ | + | 2OB1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OB1 OCA]. |
==Reference== | ==Reference== | ||
| - | A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye., Groves MR, Muller IB, Kreplin X, Muller-Dieckmann J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):526-35. Epub 2007, Mar 16. PMID:[http:// | + | A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye., Groves MR, Muller IB, Kreplin X, Muller-Dieckmann J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):526-35. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372358 17372358] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Groves, M R.]] | [[Category: Groves, M R.]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
| - | [[Category: 8- | + | [[Category: 8-an]] |
[[Category: ppm1 in the presence of 1]] | [[Category: ppm1 in the presence of 1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:57:21 2008'' |
Revision as of 15:57, 20 March 2008
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| , resolution 1.900Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | PPM1 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ppm1 with 1,8-ANS
Overview
A technique is described whereby the addition of low concentrations (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein solution prior to crystallization results in crystallization experiments in which protein crystals are strongly contrasted above background artifacts when exposed to low-intensity UV radiation. As 1,8-ANS does not covalently modify the protein sample, no further handling or purification steps are necessary. The system has been tested on a wide variety of protein samples and it has been shown that the addition of 1,8-ANS has no discernible effect on the crystallization frequencies or crystallization conditions of these proteins. As 1,8-ANS interacts with a wide variety of proteins, this is proposed to be a general solution for the automated classification of protein crystallization images and the detection of protein crystals. The results also demonstrate the expected discrimination between salt and protein crystals, as well as allowing the straightforward identification of small crystals that grow in precipitate or under a protein skin.
About this Structure
2OB1 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye., Groves MR, Muller IB, Kreplin X, Muller-Dieckmann J, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):526-35. Epub 2007, Mar 16. PMID:17372358
Page seeded by OCA on Thu Mar 20 17:57:21 2008
