2odc
From Proteopedia
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| - | [[Image:2odc.gif|left|200px]] | + | [[Image:2odc.gif|left|200px]] |
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| - | '''LEM-domain of the nuclear envelope protein emerin''' | + | {{Structure |
| + | |PDB= 2odc |SIZE=350|CAPTION= <scene name='initialview01'>2odc</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= EMD, EDMD, STA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''LEM-domain of the nuclear envelope protein emerin''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ODC is a [ | + | 2ODC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ODC OCA]. |
==Reference== | ==Reference== | ||
| - | Solution NMR structure of the barrier-to-autointegration factor-Emerin complex., Cai M, Huang Y, Suh JY, Louis JM, Ghirlando R, Craigie R, Clore GM, J Biol Chem. 2007 May 11;282(19):14525-35. Epub 2007 Mar 13. PMID:[http:// | + | Solution NMR structure of the barrier-to-autointegration factor-Emerin complex., Cai M, Huang Y, Suh JY, Louis JM, Ghirlando R, Craigie R, Clore GM, J Biol Chem. 2007 May 11;282(19):14525-35. Epub 2007 Mar 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355960 17355960] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Clore, G M.]] | [[Category: Clore, G M.]] | ||
[[Category: inner nuclear membrane protein]] | [[Category: inner nuclear membrane protein]] | ||
| - | [[Category: lem-domain multidimensional nmr dipolar | + | [[Category: lem-domain multidimensional nmr dipolar coupling]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:58:10 2008'' |
Revision as of 15:58, 20 March 2008
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| Gene: | EMD, EDMD, STA (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LEM-domain of the nuclear envelope protein emerin
Contents |
Overview
The barrier-to-autointegration factor BAF binds to the LEM domain (Em(LEM)) of the nuclear envelope protein emerin and plays an essential role in the nuclear architecture of metazoan cells. In addition, the BAF(2) dimer bridges and compacts double-stranded DNA nonspecifically via two symmetry-related DNA binding sites. In this article we present biophysical and structural studies on a complex of BAF(2) and Em(LEM). Light scattering, analytical ultracentrifugation, and NMR indicate a stoichiometry of one molecule of Em(LEM) bound per BAF(2) dimer. The equilibrium dissociation constant (K(d)) for the interaction of the BAF(2) dimer and Em(LEM), determined by isothermal titration calorimetry, is 0.59 +/- 0.03 microm. Z-exchange spectroscopy between corresponding cross-peaks of the magnetically non-equivalent subunits of the BAF(2) dimer in the complex yields a dissociation rate constant of 78 +/- 2s(-1). The solution NMR structure of the BAF(2)-Em(LEM) complex reveals that the LEM and DNA binding sites on BAF(2) are non-overlapping and that both subunits of the BAF(2) dimer contribute approximately equally to the Em(LEM) binding site. The relevance of the implications of the structural and biophysical data on the complex in the context of the interaction between the BAF(2) dimer and Em(LEM) at the nuclear envelope is discussed.
Disease
Known diseases associated with this structure: Emery-Dreifuss muscular dystrophy OMIM:[300384]
About this Structure
2ODC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution NMR structure of the barrier-to-autointegration factor-Emerin complex., Cai M, Huang Y, Suh JY, Louis JM, Ghirlando R, Craigie R, Clore GM, J Biol Chem. 2007 May 11;282(19):14525-35. Epub 2007 Mar 13. PMID:17355960
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