4jd6
From Proteopedia
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| - | + | ==Crystal structure of Mycobacterium tuberculosis Eis in complex with coenzyme A and tobramycin== | |
| - | + | <StructureSection load='4jd6' size='340' side='right' caption='[[4jd6]], [[Resolution|resolution]] 3.50Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[4jd6]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_sp._h37rv Mycobacterium sp. h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JD6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4JD6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RVBD_2416c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 Mycobacterium sp. H37Rv])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4jd6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jd6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4jd6 RCSB], [http://www.ebi.ac.uk/pdbsum/4jd6 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A recently discovered cause of tuberculosis resistance to a drug of last resort, the aminoglycoside kanamycin, results from modification of this drug by the enhanced intracellular survival (Eis) protein. Eis is a structurally and functionally unique acetyltransferase with an unusual capability of acetylating aminoglycosides at multiple positions. The extent of this regioversatility and its defining protein features are unclear. Herein, we determined the positions and order of acetylation of five aminoglycosides by NMR spectroscopy. This analysis revealed unprecedented acetylation of the 3''-amine of kanamycin, amikacin, and tobramycin, and the gamma-amine of the 4-amino-2-hydroxybutyryl group of amikacin. A crystal structure of Eis in complex with coenzyme A and tobramycin revealed how tobramycin can be accommodated in the Eis active site in two binding modes, consistent with its diacetylation. These studies, describing chemical and structural details of acetylation, will guide future efforts towards designing aminoglycosides and Eis inhibitors to overcome resistance in tuberculosis. | ||
| - | + | Chemical and Structural Insights into the Regioversatility of the Aminoglycoside Acetyltransferase Eis.,Houghton JL, Biswas T, Chen W, Tsodikov OV, Garneau-Tsodikova S Chembiochem. 2013 Sep 17. doi: 10.1002/cbic.201300359. PMID:24106131<ref>PMID:24106131</ref> | |
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| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mycobacterium sp. h37rv]] | [[Category: Mycobacterium sp. h37rv]] | ||
| - | [[Category: Biswas, T | + | [[Category: Biswas, T]] |
| - | [[Category: Chen, W | + | [[Category: Chen, W]] |
| - | [[Category: Garneau-Tsodikova, S | + | [[Category: Garneau-Tsodikova, S]] |
| - | [[Category: Tsodikov, O V | + | [[Category: Tsodikov, O V]] |
[[Category: Aminoglycoside acetyltransferase]] | [[Category: Aminoglycoside acetyltransferase]] | ||
[[Category: Gnat]] | [[Category: Gnat]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 09:26, 20 January 2015
Crystal structure of Mycobacterium tuberculosis Eis in complex with coenzyme A and tobramycin
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