2oix
From Proteopedia
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| - | [[Image:2oix.jpg|left|200px]] | + | [[Image:2oix.jpg|left|200px]] |
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| - | '''Xanthomonas XopD C470A Mutant''' | + | {{Structure |
| + | |PDB= 2oix |SIZE=350|CAPTION= <scene name='initialview01'>2oix</scene>, resolution 1.80Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= xopD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=456327 Xanthomonas euvesicatoria]) | ||
| + | }} | ||
| + | |||
| + | '''Xanthomonas XopD C470A Mutant''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OIX is a [ | + | 2OIX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xanthomonas_euvesicatoria Xanthomonas euvesicatoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OIX OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases., Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K, J Biol Chem. 2007 Mar 2;282(9):6773-82. Epub 2007 Jan 3. PMID:[http:// | + | Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases., Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K, J Biol Chem. 2007 Mar 2;282(9):6773-82. Epub 2007 Jan 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17204475 17204475] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Xanthomonas euvesicatoria]] | [[Category: Xanthomonas euvesicatoria]] | ||
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[[Category: type iii secreted effector]] | [[Category: type iii secreted effector]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:00:24 2008'' |
Revision as of 16:00, 20 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | xopD (Xanthomonas euvesicatoria) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Xanthomonas XopD C470A Mutant
Overview
XopD (Xanthomonas outer protein D), a type III secreted effector from Xanthomonas campestris pv. vesicatoria, is a desumoylating enzyme with strict specificity for its plant small ubiquitin-like modifier (SUMO) substrates. Based on SUMO sequence alignments and peptidase assays with various plant, yeast, and mammalian SUMOs, we identified residues in SUMO that contribute to XopD/SUMO recognition. Further predictions regarding the enzyme/substrate specificity were made by solving the XopD crystal structure. By incorporating structural information with sequence alignments and enzyme assays, we were able to elucidate determinants of the rigid SUMO specificity exhibited by the Xanthomonas virulence factor XopD.
About this Structure
2OIX is a Single protein structure of sequence from Xanthomonas euvesicatoria. Full crystallographic information is available from OCA.
Reference
Structural analysis of Xanthomonas XopD provides insights into substrate specificity of ubiquitin-like protein proteases., Chosed R, Tomchick DR, Brautigam CA, Mukherjee S, Negi VS, Machius M, Orth K, J Biol Chem. 2007 Mar 2;282(9):6773-82. Epub 2007 Jan 3. PMID:17204475
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