2okh
From Proteopedia
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| - | [[Image:2okh.gif|left|200px]] | + | [[Image:2okh.gif|left|200px]] |
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| - | '''Crystal structure of dimeric form of PfFabZ in crystal form3''' | + | {{Structure |
| + | |PDB= 2okh |SIZE=350|CAPTION= <scene name='initialview01'>2okh</scene>, resolution 3.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= fabZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of dimeric form of PfFabZ in crystal form3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OKH is a [ | + | 2OKH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OKH OCA]. |
==Reference== | ==Reference== | ||
| - | Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:[http:// | + | Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372349 17372349] |
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: plasmodium]] | [[Category: plasmodium]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:00:51 2008'' |
Revision as of 16:00, 20 March 2008
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| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | fabZ (Plasmodium falciparum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of dimeric form of PfFabZ in crystal form3
Overview
The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences.
About this Structure
2OKH is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum., Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K, Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349
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