4ien

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{{STRUCTURE_4ien| PDB=4ien | SCENE= }}
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==Crystal Structure of Acyl-CoA Hydrolase from Neisseria meningitidis FAM18==
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===Crystal Structure of Acyl-CoA Hydrolase from Neisseria meningitidis FAM18===
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<StructureSection load='4ien' size='340' side='right' caption='[[4ien]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_24192375}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4ien]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimf Neimf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IEN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IEN FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NMC1417 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272831 NEIMF])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acyl-CoA_hydrolase Acyl-CoA hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.2.20 3.1.2.20] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ien FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ien OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ien RCSB], [http://www.ebi.ac.uk/pdbsum/4ien PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neisseria meningitidis is the causative microorganism of many human diseases, including bacterial meningitis; together with Streptococcus pneumoniae, it accounts for approximately 80% of bacterial meningitis infections. The emergence of antibiotic-resistant strains of N. meningitidis has created a strong urgency for the development of new therapeutics, and the high-resolution structural elucidation of enzymes involved in cell metabolism represents a platform for drug development. Acetyl-CoA hydrolase is involved in multiple functions in the bacterial cell, including membrane synthesis, fatty-acid and lipid metabolism, gene regulation and signal transduction. Here, the first recombinant protein expression, purification and crystallization of a hexameric acetyl-CoA hydrolase from N. meningitidis are reported. This protein was crystallized using the hanging-drop vapour-diffusion technique at pH 8.5 and 290 K using ammonium phosphate as a precipitant. Optimized crystals diffracted to 2.0 A resolution at the Australian Synchrotron and belonged to space group P2(1)3 (unit-cell parameters a = b = c = 152.2 A), with four molecules in the asymmetric unit.
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==About this Structure==
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Expression, purification and crystallization of acetyl-CoA hydrolase from Neisseria meningitidis.,Khandokar YB, Londhe A, Patil S, Forwood JK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1303-6. doi:, 10.1107/S1744309113028042. Epub 2013 Oct 30. PMID:24192375<ref>PMID:24192375</ref>
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[[4ien]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Neimf Neimf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IEN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024192375</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Acyl-CoA hydrolase]]
[[Category: Acyl-CoA hydrolase]]
[[Category: Neimf]]
[[Category: Neimf]]
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[[Category: Forwood, J K.]]
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[[Category: Forwood, J K]]
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[[Category: Khandokar, Y B.]]
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[[Category: Khandokar, Y B]]
[[Category: Hot dog fold]]
[[Category: Hot dog fold]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 10:04, 20 January 2015

Crystal Structure of Acyl-CoA Hydrolase from Neisseria meningitidis FAM18

4ien, resolution 2.00Å

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