2olo

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[[Image:2olo.jpg|left|200px]]<br /><applet load="2olo" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2olo.jpg|left|200px]]
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caption="2olo, resolution 1.900&Aring;" />
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'''NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution'''<br />
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{{Structure
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|PDB= 2olo |SIZE=350|CAPTION= <scene name='initialview01'>2olo</scene>, resolution 1.900&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=6PC:PYRIDINE-2-CARBOXYLIC+ACID'>6PC</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>
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|ACTIVITY=
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|GENE= nikD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1932 Streptomyces tendae])
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}}
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'''NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2OLO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_tendae Streptomyces tendae] with <scene name='pdbligand=FAD:'>FAD</scene>, <scene name='pdbligand=6PC:'>6PC</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLO OCA].
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2OLO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_tendae Streptomyces tendae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OLO OCA].
==Reference==
==Reference==
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NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: structures of closed and open forms at 1.15 and 1.90 A resolution., Carrell CJ, Bruckner RC, Venci D, Zhao G, Jorns MS, Mathews FS, Structure. 2007 Aug;15(8):928-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17697998 17697998]
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NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: structures of closed and open forms at 1.15 and 1.90 A resolution., Carrell CJ, Bruckner RC, Venci D, Zhao G, Jorns MS, Mathews FS, Structure. 2007 Aug;15(8):928-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17697998 17697998]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces tendae]]
[[Category: Streptomyces tendae]]
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[[Category: rossmann fold]]
[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:19:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:01:22 2008''

Revision as of 16:01, 20 March 2008

Image:2olo.jpg


PDB ID 2olo

Drag the structure with the mouse to rotate
, resolution 1.900Å
Ligands: , and
Gene: nikD (Streptomyces tendae)
Coordinates: save as pdb, mmCIF, xml



NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.9A resolution


Overview

NikD is an unusual amino-acid-oxidizing enzyme that contains covalently bound FAD, catalyzes a 4-electron oxidation of piperideine-2-carboxylic acid to picolinate, and plays a critical role in the biosynthesis of nikkomycin antibiotics. Crystal structures of closed and open forms of nikD, a two-domain enzyme, have been determined to resolutions of 1.15 and 1.9 A, respectively. The two forms differ by an 11 degrees rotation of the catalytic domain with respect to the FAD-binding domain. The active site is inaccessible to solvent in the closed form; an endogenous ligand, believed to be picolinate, is bound close to and parallel with the flavin ring, an orientation compatible with redox catalysis. The active site is solvent accessible in the open form, but the picolinate ligand is approximately perpendicular to the flavin ring and a tryptophan is stacked above the flavin ring. NikD also contains a mobile cation binding loop.

About this Structure

2OLO is a Single protein structure of sequence from Streptomyces tendae. Full crystallographic information is available from OCA.

Reference

NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: structures of closed and open forms at 1.15 and 1.90 A resolution., Carrell CJ, Bruckner RC, Venci D, Zhao G, Jorns MS, Mathews FS, Structure. 2007 Aug;15(8):928-41. PMID:17697998

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