3q9t
From Proteopedia
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| - | + | ==Crystal structure analysis of formate oxidase== | |
| - | === | + | <StructureSection load='3q9t' size='340' side='right' caption='[[3q9t]], [[Resolution|resolution]] 2.24Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3q9t]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae_rib40 Aspergillus oryzae rib40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q9T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q9T FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAY:[(2R,3S,4R,5R)-5-(6-AMINO-9H-PURIN-9-YL)-3,4-DIHYDROXYTETRAHYDROFURAN-2-YL]METHYL+(2R,3S,4S)-5-(8-FORMYL-7-METHYL-2,4-DIOXO-3,4-DIHYDROBENZO[G]PTERIDIN-10(2H)-YL)-2,3,4-TRIHYDROXYPENTYL+DIHYDROGEN+DIPHOSPHATE'>FAY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO090012000349 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=510516 Aspergillus oryzae RIB40])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q9t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q9t RCSB], [http://www.ebi.ac.uk/pdbsum/3q9t PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Formate oxidase of Aspergillus oryzae RIB40 contains an 8-replaced FAD with molecular mass of 799 as cofactor. The (1)H-NMR spectrum of the cofactor fraction obtained from the enzyme indicated that the 8-replaced FAD in the fraction was 8-formyl-FAD, present in open form and hemiacetal form. The oxidation-reduction potentials of the open and hemiacetal forms were estimated by cyclic voltammetry to be -47 and -177 mV vs. Normal Hydrogen Electrode respectively. The structure of the enzyme was constructed using diffraction data to 2.24 A resolution collected from a crystal of the enzyme. His(511) and Arg(554) were situated close to the pyrimidine part of the isoalloxazine ring of 8-formyl-FAD in open form. The enzyme had 8-formyl-FAD, the oxidation potential of which was approximately 160 mV more positive than that of FAD, and the His-Arg pair at the catalytic site, unlike the other enzymes belonging to the glucose-methanol-choline oxidoreductase family. | ||
| - | + | Formate Oxidase, an Enzyme of the Glucose-Methanol-Choline Oxidoreductase Family, Has a His-Arg Pair and 8-Formyl-FAD at the Catalytic Site.,Doubayashi D, Ootake T, Maeda Y, Oki M, Tokunaga Y, Sakurai A, Nagaosa Y, Mikami B, Uchida H Biosci Biotechnol Biochem. 2011 Sep 7. PMID:21897046<ref>PMID:21897046</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Aspergillus oryzae rib40]] | [[Category: Aspergillus oryzae rib40]] | ||
| - | [[Category: Doubayashi, D | + | [[Category: Doubayashi, D]] |
| - | [[Category: Maeda, Y | + | [[Category: Maeda, Y]] |
| - | [[Category: Mikami, B | + | [[Category: Mikami, B]] |
| - | [[Category: Nagaosa, Y | + | [[Category: Nagaosa, Y]] |
| - | [[Category: Oki, M | + | [[Category: Oki, M]] |
| - | [[Category: Ootake, T | + | [[Category: Ootake, T]] |
| - | [[Category: Sakurai, A | + | [[Category: Sakurai, A]] |
| - | [[Category: Tokunaga, Y | + | [[Category: Tokunaga, Y]] |
| - | [[Category: Uchida, H | + | [[Category: Uchida, H]] |
[[Category: 8-formyl-fad]] | [[Category: 8-formyl-fad]] | ||
[[Category: Formate oxidase]] | [[Category: Formate oxidase]] | ||
[[Category: Glucose-methanol-choline oxidoreductase family]] | [[Category: Glucose-methanol-choline oxidoreductase family]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
Revision as of 10:39, 20 January 2015
Crystal structure analysis of formate oxidase
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