4k8x
From Proteopedia
(Difference between revisions)
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| - | + | ==Binary complex of 9N DNA polymerase in the replicative state== | |
| - | + | <StructureSection load='4k8x' size='340' side='right' caption='[[4k8x]], [[Resolution|resolution]] 2.28Å' scene=''> | |
| - | { | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[4k8x]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermococcus_sp._9on-7 Thermococcus sp. 9on-7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K8X FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CY:1-(3-HYDROXYPROPYL)-2-{(1E,3E,5E)-5-[1-(3-HYDROXYPROPYL)-3,3-DIMETHYL-1,3-DIHYDRO-2H-INDOL-2-YLIDENE]PENTA-1,3-DIEN-1-YL}-3,3-DIMETHYL-3H-INDOLIUM'>5CY</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DOC:2,3-DIDEOXYCYTIDINE-5-MONOPHOSPHATE'>DOC</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qht|1qht]], [[4k8z|4k8z]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol, polA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103799 Thermococcus sp. 9oN-7])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k8x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k8x RCSB], [http://www.ebi.ac.uk/pdbsum/4k8x PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Replicate it: Structures of KOD and 9 degrees N DNA polymerases, two enzymes that are widely used to replicate DNA with highly modified nucleotides, were solved at high resolution in complex with primer/template duplex. The data elucidate substrate interaction of the two enzymes and pave the way for further optimisation of the enzymes and substrates. | ||
| - | + | Structures of KOD and 9 degrees N DNA Polymerases Complexed with Primer Template Duplex.,Bergen K, Betz K, Welte W, Diederichs K, Marx A Chembiochem. 2013 Jun 17;14(9):1058-62. doi: 10.1002/cbic.201300175. Epub 2013, Jun 3. PMID:23733496<ref>PMID:23733496</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | |||
| + | ==See Also== | ||
| + | *[[DNA polymerase|DNA polymerase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Thermococcus sp. 9on-7]] | [[Category: Thermococcus sp. 9on-7]] | ||
| - | [[Category: Betz, K | + | [[Category: Betz, K]] |
| - | [[Category: Diederichs, K | + | [[Category: Diederichs, K]] |
| - | [[Category: Marx, A | + | [[Category: Marx, A]] |
[[Category: Binary complex]] | [[Category: Binary complex]] | ||
[[Category: Dna polymerase]] | [[Category: Dna polymerase]] | ||
[[Category: Transferase-dna complex]] | [[Category: Transferase-dna complex]] | ||
Revision as of 11:07, 20 January 2015
Binary complex of 9N DNA polymerase in the replicative state
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