2oqc

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[[Image:2oqc.jpg|left|200px]]<br /><applet load="2oqc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2oqc.jpg|left|200px]]
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caption="2oqc, resolution 2.50&Aring;" />
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'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''<br />
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{{Structure
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|PDB= 2oqc |SIZE=350|CAPTION= <scene name='initialview01'>2oqc</scene>, resolution 2.50&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11]
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|GENE= yxeI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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}}
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'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2OQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Active as [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].
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2OQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].
==Reference==
==Reference==
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Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511127 16511127]
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Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511127 16511127]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Penicillin amidase]]
[[Category: Penicillin amidase]]
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[[Category: Sivaraman, H.]]
[[Category: Sivaraman, H.]]
[[Category: Suresh, C G.]]
[[Category: Suresh, C G.]]
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[[Category: bacillus subtilis]]
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[[Category: bacillus subtili]]
[[Category: choloylglycine hydrolase]]
[[Category: choloylglycine hydrolase]]
[[Category: conjugated bile acid hydrolase]]
[[Category: conjugated bile acid hydrolase]]
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[[Category: penicillin v acylase]]
[[Category: penicillin v acylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:21:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:03:07 2008''

Revision as of 16:03, 20 March 2008

Image:2oqc.jpg


PDB ID 2oqc

Drag the structure with the mouse to rotate
, resolution 2.50Å
Gene: yxeI (Bacillus subtilis)
Activity: Penicillin amidase, with EC number 3.5.1.11
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Penicillin V acylase from Bacillus subtilis


Overview

Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.

About this Structure

2OQC is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:16511127

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