2oqp
From Proteopedia
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| - | [[Image:2oqp.jpg|left|200px]] | + | [[Image:2oqp.jpg|left|200px]] |
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| - | '''Solution structure of human interleukin-21''' | + | {{Structure |
| + | |PDB= 2oqp |SIZE=350|CAPTION= <scene name='initialview01'>2oqp</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= IL21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''Solution structure of human interleukin-21''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OQP is a [ | + | 2OQP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQP OCA]. |
==Reference== | ==Reference== | ||
| - | The existence of multiple conformers of interleukin-21 directs engineering of a superpotent analogue., Bondensgaard K, Breinholt J, Madsen D, Omkvist DH, Kang L, Worsaae A, Becker P, Schiodt CB, Hjorth SA, J Biol Chem. 2007 Aug 10;282(32):23326-36. Epub 2007 Jun 12. PMID:[http:// | + | The existence of multiple conformers of interleukin-21 directs engineering of a superpotent analogue., Bondensgaard K, Breinholt J, Madsen D, Omkvist DH, Kang L, Worsaae A, Becker P, Schiodt CB, Hjorth SA, J Biol Chem. 2007 Aug 10;282(32):23326-36. Epub 2007 Jun 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17565991 17565991] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: cytokine]] | [[Category: cytokine]] | ||
[[Category: four helix bundle]] | [[Category: four helix bundle]] | ||
| - | [[Category: multiple | + | [[Category: multiple conformer]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:03:14 2008'' |
Revision as of 16:03, 20 March 2008
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| Gene: | IL21 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution structure of human interleukin-21
Overview
The high resolution three-dimensional structure of human interleukin (hIL)-21 has been resolved by heteronuclear NMR spectroscopy. Overall, the hIL-21 structure is dominated by a well defined central four-helical bundle, arranged in an up-up-down-down topology, as observed for other cytokines. A segment of the hIL-21 molecule that includes the third helical segment, helix C, is observed to exist in two distinct and interchangeable states. In one conformer, the helix C segment is presented in a regular, alpha-helical conformation, whereas in the other conformer, this segment is largely disordered. A structure-based sequence alignment of hIL-21 with receptor complexes of the related cytokines, interleukin-2 and -4, implied that this particular segment is involved in receptor binding. An hIL-21 analog was designed to stabilize the region around helix C through the introduction of a segment grafted from hIL-4. This novel hIL-21 analog was demonstrated to exhibit a 10-fold increase in potency in a cellular assay.
About this Structure
2OQP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The existence of multiple conformers of interleukin-21 directs engineering of a superpotent analogue., Bondensgaard K, Breinholt J, Madsen D, Omkvist DH, Kang L, Worsaae A, Becker P, Schiodt CB, Hjorth SA, J Biol Chem. 2007 Aug 10;282(32):23326-36. Epub 2007 Jun 12. PMID:17565991
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