2or1
From Proteopedia
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| - | [[Image:2or1.jpg|left|200px]] | + | [[Image:2or1.jpg|left|200px]] |
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| - | '''RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION''' | + | {{Structure |
| + | |PDB= 2or1 |SIZE=350|CAPTION= <scene name='initialview01'>2or1</scene>, resolution 2.500Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OR1 is a [ | + | 2OR1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_434 Bacteriophage 434]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OR1 OCA]. |
==Reference== | ==Reference== | ||
| - | Recognition of a DNA operator by the repressor of phage 434: a view at high resolution., Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC, Science. 1988 Nov 11;242(4880):899-907. PMID:[http:// | + | Recognition of a DNA operator by the repressor of phage 434: a view at high resolution., Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC, Science. 1988 Nov 11;242(4880):899-907. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3187531 3187531] |
[[Category: Bacteriophage 434]] | [[Category: Bacteriophage 434]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:03:22 2008'' |
Revision as of 16:03, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION
Overview
The repressors of temperate bacteriophages such as 434 and lambda control transcription by binding to a set of DNA operator sites. The different affinity of repressor for each of these sites ensures efficient regulation. High-resolution x-ray crystallography was used to study the DNA-binding domain of phage 434 repressor in complex with a synthetic DNA operator. The structure shows recognition of the operator by direct interactions with base pairs in the major groove, combined with the sequence-dependent ability of DNA to adopt the required conformation on binding repressor. In particular, a network of three-centered bifurcated hydrogen bonds among base pairs in the operator helps explain why 434 repressor prefers certain sites over others. These bonds, which stabilize the conformation of the bound DNA, can form only with certain sequences.
About this Structure
2OR1 is a Single protein structure of sequence from Bacteriophage 434. Full crystallographic information is available from OCA.
Reference
Recognition of a DNA operator by the repressor of phage 434: a view at high resolution., Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC, Science. 1988 Nov 11;242(4880):899-907. PMID:3187531
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