4hfm
From Proteopedia
(Difference between revisions)
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| - | [[ | + | ==X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum== |
| + | <StructureSection load='4hfm' size='340' side='right' caption='[[4hfm]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hfm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFM FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hfj|4hfj]], [[4hfn|4hfn]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NtADH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 Nicotiana tabacum])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/2-alkenal_reductase 2-alkenal reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.74 1.3.1.74] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hfm RCSB], [http://www.ebi.ac.uk/pdbsum/4hfm PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/Q9SLN8_TOBAC Q9SLN8_TOBAC]] Reduces the C=C double bonds of alpha, beta unsaturated enones, but has no activity on enones with an endocyclic C=C double-bond. Shows a high specificity for NADPH as the hybrid donor. Substrates are 1-nitrocyclohexene, 2-methylpentenal, trans-cinnamaldehyde, methyl-trans-2-methylcinnamaldehyde, trans-2-nonenal and 1-octen-3-one. Reduced activity with aplha-methyl transcinnamaldehyde, 1-cyclohexene-1-carboxaldehyde, methyl crotonate, (R)-pulegone, and dimethyl itaconate and no activity with maleimides, citral, (5R)- or (5S)-carvone, (S)-perillyl alcohol, and substituted cyclohexenones and cyclopentenones (PubMed:17945329, Ref.3). May also act as a allyl-alcohol dehydrogenase by catalyzing the dehydrogenation of secondary allylic alcohols rather than saturated secondary alcohols. Allyl-alcohol dehydrogenase is specific for the S-stereoisomer of the alcohols (PubMed:11117876).<ref>PMID:11117876</ref> <ref>PMID:17945329</ref> [REFERENCE:3] | ||
| - | + | ==See Also== | |
| - | + | *[[Alcohol dehydrogenase|Alcohol dehydrogenase]] | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | == | + | </StructureSection> |
| - | + | ||
[[Category: 2-alkenal reductase]] | [[Category: 2-alkenal reductase]] | ||
[[Category: Nicotiana tabacum]] | [[Category: Nicotiana tabacum]] | ||
| - | [[Category: Scrutton, N S | + | [[Category: Scrutton, N S]] |
| - | [[Category: Toogood, H S | + | [[Category: Toogood, H S]] |
[[Category: Alkene reduction]] | [[Category: Alkene reduction]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Rossmann fold]] | [[Category: Rossmann fold]] | ||
[[Category: Twisted b-barrel]] | [[Category: Twisted b-barrel]] | ||
Revision as of 11:54, 20 January 2015
X-ray Crystal Structure of a NADP(H)-bound Double Bond Reductase from Nicotiana tabacum
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