2otj

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[[Image:2otj.jpg|left|200px]]<br /><applet load="2otj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2otj.jpg|left|200px]]
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caption="2otj, resolution 2.900&Aring;" />
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'''13-deoxytedanolide bound to the large subunit of Haloarcula marismortui'''<br />
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{{Structure
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|PDB= 2otj |SIZE=350|CAPTION= <scene name='initialview01'>2otj</scene>, resolution 2.900&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=13T:13-DEOXYTEDANOLIDE'>13T</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''13-deoxytedanolide bound to the large subunit of Haloarcula marismortui'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2OTJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui] with <scene name='pdbligand=13T:'>13T</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=K:'>K</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTJ OCA].
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2OTJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTJ OCA].
==Reference==
==Reference==
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The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17321546 17321546]
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The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17321546 17321546]
[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: NA]]
[[Category: NA]]
[[Category: 13 deoxytedanolide]]
[[Category: 13 deoxytedanolide]]
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[[Category: 50s]]
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[[Category: 50]]
[[Category: antibiotic complex]]
[[Category: antibiotic complex]]
[[Category: ribosome]]
[[Category: ribosome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:22:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:04:16 2008''

Revision as of 16:04, 20 March 2008

Image:2otj.jpg


PDB ID 2otj

Drag the structure with the mouse to rotate
, resolution 2.900Å
Ligands: , , , , and
Coordinates: save as pdb, mmCIF, xml



13-deoxytedanolide bound to the large subunit of Haloarcula marismortui


Overview

Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria.

About this Structure

2OTJ is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

Reference

The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:17321546

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