2otl
From Proteopedia
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| - | [[Image:2otl.jpg|left|200px]] | + | [[Image:2otl.jpg|left|200px]] |
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| - | '''Girodazole bound to the large subunit of Haloarcula marismortui''' | + | {{Structure |
| + | |PDB= 2otl |SIZE=350|CAPTION= <scene name='initialview01'>2otl</scene>, resolution 2.700Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GIR:GIRODAZOLE'>GIR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Girodazole bound to the large subunit of Haloarcula marismortui''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2OTL is a [ | + | 2OTL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OTL OCA]. |
==Reference== | ==Reference== | ||
| - | The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:[http:// | + | The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17321546 17321546] |
[[Category: Haloarcula marismortui]] | [[Category: Haloarcula marismortui]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: MG]] | [[Category: MG]] | ||
[[Category: NA]] | [[Category: NA]] | ||
| - | [[Category: | + | [[Category: 50]] |
[[Category: antibiotic complex]] | [[Category: antibiotic complex]] | ||
[[Category: girodazole]] | [[Category: girodazole]] | ||
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[[Category: ribosome]] | [[Category: ribosome]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:04:17 2008'' |
Revision as of 16:04, 20 March 2008
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| , resolution 2.700Å | |||||||
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| Ligands: | , , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Girodazole bound to the large subunit of Haloarcula marismortui
Overview
Crystal structures of the 50 S ribosomal subunit from Haloarcula marismortui complexed with two antibiotics have identified new sites at which antibiotics interact with the ribosome and inhibit protein synthesis. 13-Deoxytedanolide binds to the E site of the 50 S subunit at the same location as the CCA of tRNA, and thus appears to inhibit protein synthesis by competing with deacylated tRNAs for E site binding. Girodazole binds near the E site region, but is somewhat buried and may inhibit tRNA binding by interfering with conformational changes that occur at the E site. The specificity of 13-deoxytedanolide for eukaryotic ribosomes is explained by its extensive interactions with protein L44e, which is an E site component of archaeal and eukaryotic ribosomes, but not of eubacterial ribosomes. In addition, protein L28, which is unique to the eubacterial E site, overlaps the site occupied by 13-deoxytedanolide, precluding its binding to eubacterial ribosomes. Girodazole is specific for eukarytes and archaea because it makes interactions with L15 that are not possible in eubacteria.
About this Structure
2OTL is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.
Reference
The structures of antibiotics bound to the E site region of the 50 S ribosomal subunit of Haloarcula marismortui: 13-deoxytedanolide and girodazole., Schroeder SJ, Blaha G, Tirado-Rives J, Steitz TA, Moore PB, J Mol Biol. 2007 Apr 13;367(5):1471-9. Epub 2007 Feb 7. PMID:17321546
Page seeded by OCA on Thu Mar 20 18:04:17 2008
Categories: Haloarcula marismortui | Protein complex | Blaha, G. | Schroeder, S J. | Tirado-Rives, J. | CD | CL | GIR | K | MG | NA | 50 | Antibiotic complex | Girodazole | Girolline | Large subunit | Ribosome
